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Beaumont Laboratory

Histoplasma Antigen

MiraVista #310, BAL: EPIC: LAB6984, SOFT: XHIAB, CSF: EPIC: LAB6985, SOFT: XHIAC, Serum: EPIC: LAB6497, SOFT: XHIAS, Urine: EPIC: LAB6496, SOFT: XHIAU

Specimen Collection Criteria

Collect: One Gold-top SST tube or plain Red-top tube.
Also acceptable: Urine, Bronchoalveolar Lavage (BAL) or Cerebrospinal Fluid (CSF) in a sterile container.

Physician Office/Drawsite Specimen Preparation

Let blood specimens clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F). Maintain BAL, CSF, and urine specimens refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: 2.0 mL serum, BAL, CSF, or urine, refrigerated (2-8°C or 36-46°F). (Min: 1.2 mL serum, 0.8 mL CSF, and 0.5 mL BAL/Urine)

Rejection Criteria

  • Specimens not collected and processed as indicated.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 14 days
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): Indefinite

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.


Sent to MiraVista Diagnostics, Indianapolis, IN.


Monday - Friday.
Results available within 2-4 days.

Reference Range

None detected.

Results are reported as a numerical value which are interpreted as positive or negative.

Test Methodology

Quantitative Sandwich Enzyme Immunoassay (EIA).


Specificity of at least 98% (1).
Sensitivity varies with type of histoplasmosis (1):

  • Disseminated - 92%
  • Acute pulmonary - 44%
  • Histopasma meningitis - 67%

Antigen can be detected in the urine of 92% and the serum of 82% of patients with disseminated disease. For patients with disseminated cases of disease with meningeal or pulmonary involvement, antigen can also be detected in the cerebrspinal fluid of 40 % and bronchoalveolar lavage fluid of 70% of patients, respectively. Antigen clears with treatment and increases with relapse, so antigen levels provide assistance in monitoring the effectiveness of drug therapy. (2)

Clinical Utility

This assay aids in the diagnosis of histoplasmosis and in monitoring the response to treatment.


  1. Diagnosis of histoplasmosis by antigen detection. N Engl J Med 1986; 314:83.
  2. Durkin M, Connolly P, Wheat J. Comparison of Radioimmunoassay and Enzyme-Linked Immunoassay Methods for Detection of Histoplasma capsulatum var. capsulatum Antigen. Journal of Clinical Microbiology 1997; 35(9):2252-2255.

CPT Code


Test Codes

MiraVista #310, BAL: EPIC: LAB6984, SOFT: XHIAB, CSF: EPIC: LAB6985, SOFT: XHIAC, Serum: EPIC: LAB6497, SOFT: XHIAS, Urine: EPIC: LAB6496, SOFT: XHIAU

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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.