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Beaumont Laboratory

Vitamin B2 Level

Riboflavin, Vitamin B2, MAYO: VITB2, EPIC: LAB6179, SOFT: XVTB2


The patient must be fasting 12-14 hours prior to specimen collection (for infants, collect specimen just prior to next schedule feeding).

Specimen Collection Criteria

Collect: One Dark Green-top Lithium or Sodium Heparin tube.

  • Protect specimen from light.
  • Send specimen for processing immediately after collection.

Physician Office/Drawsite Specimen Preparation

Protect specimen from light. Centrifuge to separate plasma from cells. Transfer plasma to an amber plastic transport tube and freeze (-20°C/-4°F or below).

Preparation for Courier Transport

Transport: 0.5 mL light-protected plasma, frozen (-20°C/-4°F or below). (Min: 0.25 mL)

Rejection Criteria

  • Specimens not protected from light.
  • Grossly lipemic specimens.
  • Specimens not collected and processed as indicated.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.


Sent to Mayo Medical Laboratories, Rochester, MN.


Monday, Wednesday, Friday
Results available 3-5 days.

Reference Range

By report.

Test Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).


By report.

Clinical Utility

The Vitamin B2 (Riboflavin) assay aids in the evaluation of patients with ariboflavinosis and in patients who present with chronic, progressive nerve compression disorders such as carpal or tarsal tunnel syndrome.

Ariboflavinosis is the dietary deficiency of Vitamin B2 (Riboflavin). This deficiency causes a syndrome characterized by sore throat, hyperemia, and edema of the pharyngeal and oral mucous membranes, cheilitis, angular stomatitis, glossitis, corneal vascularization, dyssebacia with dermatitis, and normochromic normocytic anemia.

Deficiencies of riboflavin and vitamin B6 have been suggested as potentiating factors for chronic progressive nerve compression disorders, such as carpal tunnel syndrome.

CPT Code


Test Codes


Last Updated


Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.