Beaumont Laboratory - Lab Test Details

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Beaumont Laboratory

Haemophilus Influenzae B IgG

Haemophilus Influenzae Type b Antibody, IgG, ARUP #50542, Antrim #81050, EPIC: LAB6045, SOFT: XHAIG


"Pre" and "post" Haemophilus influenzae B vaccination samples should be submitted together for testing. "Post" samples should be drawn 30 days after immunization and must be received within 60 days of "pre" sample. Please clearly mark samples "pre-vaccine" or "post-vaccine" so that samples will be saved and tested simultaneously.

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube.

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Min: 0.15 mL)

Rejection Criteria

  • Hemolyzed specimens.
  • Severely lipemic specimens.
  • Specimens not collected and processed as indicated.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 1 year

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.


Sent to ARUP Laboratories, Salt Lake City, UT.


Sunday - Saturday.
Results available in 2-4 days.

Reference Range

Less than 1.0 mcg/mL: Antibody concentration not protective.
Greater than or equal to 1.0 mcg/mL: Antibody to Haemophilus influenzae B detected. Suggestive of protection.

Test Methodology

Quantitative Multiplex Bead Assay.


Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza B antibody, IgG as follows:

  1. If the post-vaccination concentration is less than 3.0 mcg/mL, the patient is considered to be a non-responder.
  2. If the post-vaccination concentration is greater than or equal to 3.0 mcg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a non-responder.

Clinical Utility

This assay is used to assess the production of antibody against a protein-polysaccharide conjugate vaccine.

CPT Code


Test Codes

ARUP #50542, Antrim #81050, EPIC: LAB6045, SOFT: XHAIG

Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.