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Beaumont Laboratory

Secobarbital Level

Seconal, Antrim #17012, EPIC: LAB5416, SOFT: SECOB

Specimen Collection Criteria

Collect: One plain Red-top tube. (Minimum Whole Blood: 2.0 mL)

Do not use Serum Separator Tubes.

Record the exact time of specimen collection on the tube or in the computer system.

Physician Office/Drawsite Specimen Preparation

Let specimens clot 30-60 minutes. Centrifuge specimen to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Min: 1.0 mL serum)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Min: 1.0 mL serum)

Rejection Criteria

  • Serum Separator (SST) tubes.
  • Severely lipemic or hemolyzed specimens.   


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 8 hours
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Frozen (-20°C/-4°F or below): 2 months


Royal Oak Toxicology Laboratory.


Sunday - Saturday, 24 hours a day.
STAT results (screen) available within 3 hours of receipt in the Laboratory.
Routine results available within 24 hours. 

Reference Range

Therapeutic Range: 1-5 mcg/mL.
Critical: Greater than 15 mcg/mL.

Test Methodology

Gas Liquid Chromatography with Flame Ionization Detection (GC/FID).

This test was developed and its performance characteristics determined by Beaumont Health. It has not been cleared or approved by the FDA. The Laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes and should not be regarded as investigational or for research.


Barbiturates are respiratory depressants and serum levels above the therapeutic range may indicate toxicity.

Clinical Utility

This assay is used to monitor the therapeutic drug level and evaluate the toxicity of secobarbital. Secobarbital is a short-acting barbiturate with sedative-hypnotic activity used for the short-term treatment of insomnia and as a preanesthetic.

CPT Code

80345 (alternate: G0480).

Test Codes

Antrim #17012, EPIC: LAB5416, SOFT: SECOB

Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.