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Beaumont Laboratory

HLA B27 Screen

HLA-Antigen B27, HLA-B27, HLA-B27 Screening Assay, Flow Cytometry, HLA B27 Screen, EPIC: LAB6411, SOFT: FB27G

Specimen Collection Criteria

Collect: One Lavender-top EDTA tube.
Also acceptable: One Yellow-top ACD tube or Green-top Sodium Heparin tube.

FedEx Shipping Instructions

Transport 4 mL whole blood at room temperature. Specimen must be received within 48 hours of collection.

Read our complete shipping instructions.

Physician Office/Drawsite Specimen Preparation

Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Specimens must be received in the Laboratory within 48 hours of collection.

Preparation for Courier Transport

Transport: Whole blood, at room temperature (20-25°C or 68-77°F).

Rejection Criteria

  • Clotted specimens.
  • Specimens received greater than 48 hours post collection are acceptable with approval of the Flow Cytometry Laboratory supervisor or pathologist on service.
  • This test will only be performed once in a lifetime for a patient.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 48 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days


Royal Oak Flow Cytometry Laboratory.


Monday, Wednesday, Friday.
Results available in 1-3 days.

Reference Range

Patients will be reported as negative or positive for HLA-B27 Antigen.

Test Methodology

Immunophenotyping by Multiparameter Flow Cytometry.


This test is reported as positive or negative. If the result is in the equivocal range, the HLA test “Class I Typing for Disease Association” will be added on as a confirmatory test. A separate report will be issued. If the HLA laboratory is unable to confirm the result, the specimen will be reported as equivocal and a comment will be added: “Unable to confirm by HLA Laboratory molecular SPP testing. Recommend specimen redraw.”

For Oakwood Patients:

An equivocal result will not trigger reflex confirmatory HLA testing. The result will be reported out as “Equivocal” and the following comment will be issued:

“The anti-B27 antibody clone used in the HLA-B27 assay can cross-react with other HLA-B antigens, creating a false positive result with this screening test. Therefore, for results falling within the established equivocal range, it is recommended that genotype testing be performed to confirm the presence of true HLA-B27 expression. Please order “HLA test Class I Typing for Disease Association” to receive a confirmatory result.”

Clinical Utility

Flow cytometric lymphocyte analysis has allowed for rapid and sensitive screening for the presence of HLA-B27, which may be valuable in the evaluation of patients with arthritic symptoms, as seen in ankylosing spondylitis. HLA-B27 positivity has been found to be highly associated with ankylosing spondylitis. Testing for the presence of the HLA-B27 antigen in a patient is used to assist in the diagnosis of ankylosing spondylitis and /or determine a genetic predisposition for ankylosing spondylitis.

CPT Code


Test Codes


Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.