Beaumont Laboratory - Lab Test Details

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Beaumont Laboratory

Testosterone, Total, Females or Children

Testosterone, Females and Children, Total Testosterone, Women and Children, EPIC: LAB6725, SOFT: TESTF


This test should be ordered on females and patients ages 18 and younger and measures total testosterone by LCMS. This method has the analytical sensitivity and specificity required to quantify testosterone normally present in the serum of females or children.

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)
Also acceptable: One plain Red-top tube.

  • Contact the Laboratory for acceptability of other tube types.
  • Due to Diurnal variation, it is recommended that the specimen be collected between 6:00 - 10:00 a.m., however, it is not required and specimens collected at other times will not be rejected.

Physician Office/Drawsite Specimen Preparation

Let specimens clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection. For Red-top tubes, serum should be transferred to a plastic transport tube and refrigerated within two hours of collection. (Min: 1.0 mL serum)

Preparation for Courier Transport

Transport: Centrifuged SST tube or 2.0 mL serum, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 1.0 mL)

Rejection Criteria

  • Specimens collected with EDTA.
  • Red-top tubes with serum not separated from cells within two hours of collection.


Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 2 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days


Royal Oak Special Testing Laboratory.


Monday - Friday.
Results available in 2-4 days

Reference Range

By report.

Test Methodology

Ultra High Performance Liquid Chromatography/Tandem Mass Spectrometry (UHPLC-MS/MS).

Clinical Utility

  • Testosterone measurement in women and children has been an analytical challenge due to the relatively low concentrations observed in these populations. Further difficulties have involved concerns over the specificity of available immunoassays for testosterone, particularly at low levels (1, 2). A third issue has been the existence of testosterone in both a protein bound (presumably inactive) and an unbound (theoretically active) form.
  • Accurate measurement of total testosterone and its bound and unbound sub-fractions in women are important in the assessment of hyperandrogenic states such as hirsutism, acne, polycystic ovarian syndrome, congenital adrenal hyperplasia and androgen secreting tumors. There is additional value in the assessment of pediatric growth, precocious and delayed puberty, hypogonadism and unexplained virilization in children under the age of 18 years.
  • Testosterone measurement using LCMS/MS is designed to measure total testosterone at low levels commonly seen in women and children.


  1. Rosner W, Auchus RJ, Azziz, R, Sluss PM, Raff H. Position Statement: Utility, Limitations, and Pitfalls in Measuring Testosterone: An Endocrine Society Position Statement. JCEM 92:405-413, 2007.
  2. Taieb J, Mathian B. Millot F, Patricot M-C, Mathieu E, Queyrel N, Lacroix I, Somma Delpero C, Boudou P. Testosterone Measured by 10 Immunoassays and by Isotope-Dilution Gas Chromatography Mass-Spectrometry in Sera from 116 Men, Women, and Children. Clin Chem 49:1381-1395, 2003.

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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.