Beaumont Laboratory - Lab Test Details

Lab results are too important to go anywhere else

Beaumont Laboratory

Insulin, 2 Hour Post 75 g Glucola



Specimen Collection Criteria

Collect: One Gold-top SST tube 2 hours after the ingestion of the 75 g Glucola load.

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transfer serum to a plastic aliquot tube and refrigerate (2-8°C or 36-46°F) immediately. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Serum aliquot tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)

Rejection Criteria

  • Lipemic, icteric, or grossly hemolyzed specimens.
  • Samples left on the clot at room temperature.
  • Samples subjected to repeated freeze/thaw cycles. 


Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days


Royal Oak Special Testing Laboratory.


Monday - Friday.
Results available in two business days.

Reference Range

2 Hour Post 75 Gram Glucola: 29-93 microIU/mL.

Test Methodology

Chemiluminescence Immunoassay.


Some investigators have found the glucose to insulin ratio, 2 hours following 75 grams Glucola administration, to be a useful adjunct to screening for polycystic ovary syndrome in some patients. The results are usually used in conjunction with a fasting glucose:insulin ratio.

Patients undergoing insulin therapy form anti-insulin antibodies that are capable of interfering with this assay.

Clinical Utility

Evaluation of glucose intolerance and investigation of polycystic ovary syndrome.


  1. Legro RS, Finegood D, Dunaif A. A fasting glucose to insulin ratio is a useful measure of insulin sensitivity in women with polycystic ovary syndrome. J.Clin Endocrino Metab. 83:2694-98, 1998.

CPT Code


Test Codes


Last Updated


Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.