Beaumont Laboratory - Lab Test Details

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Beaumont Laboratory

Insulin Antibodies

Insulin Antibody, ARUP #99228, EPIC: LAB6070, SOFT: XIA

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube.

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and freeze (-20°C/-4°F or below).

Preparation for Courier Transport

Transport: 0.5 mL serum, frozen (-20°C/-4°F or below). (Min: 0.1 mL)

Rejection Criteria

  • Hemolyzed specimens.
  • Lipemic specimens.
  • Specimens not collected and processed as indicated.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 2 months

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.


Sent to ARUP Laboratories, Salt Lake City, UT.


Tuesday, Wednesday, Friday.
Results available in 3-6 days.

Reference Range

Negative: 0.4 Kronus units/mL or less.
Positive: Greater than 0.4 Kronus units/mL.
Note: Kronus units are arbitrary units. Kronus units = U/mL.

Test Methodology

Quantitative Radioimmunoassay (RIA).

Clinical Utility

This assay aids in the detection of insulin antibody in patients who inject insulin for a longer period of time, either human, pork, or beef insulin. Insulin-related peptides (impurities) are invariably present in most insulin preparations and are the major immunogenic components in insulin. IgG antibodies are formed to the peptides so that some of each new dose of insulin is complexed with these antibodies and is not available for its glucose-depressing functions. Patients require larger doses of insulin because of this insulin resistance. These insulin antibodies are also associated with "factitious hypoglycemia" where hypoglycemia is due to the injection of insulin rather than to a clinical problem such as insulinoma.

CPT Code


Test Codes

ARUP #99228, EPIC: LAB6070, SOFT: XIA

Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.