Honey Bee Venom (Allergen Specific IgE)

Honey Bee Venom (i1) , Antrim #31860, EPIC: LAB5650, SOFT: EHB

Specimen Collection Criteria

Collect: One Gold-top SST tube.

Twenty individual allergen assays or allergen screens can be performed on one 5 mL Gold-top SST tube. Each allergen assay requires 100 mcL of serum.

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection.

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Plasma specimens.
  • Severely lipemic or hemolyzed specimens.


0,Centrifuged SST tubes may be transported and stored at ambient temperatures (2o to 30o C; 36o to 86o F) for up to 48 hours. DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the gel and refrigerated (2o - 8o C) or frozen at -20o C or below.

Red-top tubes and Microtainers® may be stored at ambient temperatures for up to 16 hours and for up to 3 days at refrigerator temperatures (2o - 8o C). DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the clot and refrigerated (2o - 8o C) or frozen at - 20o C or below.

0,Serum specimens (pour-overs) may be stored at room temperature (20o to 26o C; 68o to 79o F) for up to 1 week, refrigerator temperature (2o to 8o C; 36o to 47o F) for two weeks, and at -20o C (-4o F) for up to 3 months. Specimens stored at - 70o C (-94o F) can be stored indefinitely.


Monday - Friday.
Results available the next business day.

Reference Range

Allergy Reference Range: Less than 0.35 kU/L. 

Range (kU/L) Class Interpretation
Less than or equal to 0.34 0 Negative
0.35-0.69 1 Low
0.70-3.49 2 Medium
3.50-17.49 3 High
17.50-49.99 4 Very High
50.0-100.0 5 Very High
Greater than 100 6 Very High

Test Methodology

Fluorescence Enzyme Immunoassay (FEIA).


The allergen class may not be predictive of clinical disease in some patients. The diagnosis of allergy should be based upon patient history and clinical findings. The diagnosis of allergy should not be based upon laboratory findings alone.

Clinical Utility

A positive test result (class 1 or greater) is indicative of the presence of allergen-specific IgE and suggests an increased likelihood of allergic disease.

Clinical Disease

In the United States it is estimated that one to two million people are severely allergic to stinging insect venoms. Approximately 90 to 100 deaths occur annually from sting reactions. This number may under-represent the true total because some sting reactions are misdiagnosed as heart attacks, sunstrokes, or other acute illnesses. Death can occur without a previous history of a sting allergy. The frequency of fatal reactions due to anaphylaxis increases with age.

Prophylactic measures must be taken for those individuals who develop severe reactions to insect stings. Specific immunotherapy (hyposensitization) remains the most effective means of treatment with a 96% success rate (1).


  1. Kaplan, Allen P. M.D. Allergy. Churchill Livingstone: New York, pg 508-509, 1985.

CPT Code


Test Codes

Antrim #31860, EPIC: LAB5650, SOFT: EHB

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