Hepatitis C Virus Genotype

HCV Genotyping, HCV Typing, Hep C, HCV Mutation Analysis, HCV Genotype , EPIC: LAB6530, SOFT: IHCVG

Specimen Collection Criteria

Collect: Serum OR plasma.

  • Serum: One Gold-top SST or plain Red-top tube.
  • Plasma: One Lavender-top EDTA tube.
  • Minimum volume and pediatric minimum 1.5 mL serum or plasma.
  • Separate specimens must be submitted when multiple tests are ordered. 

Physician Office/Drawsite Specimen Preparation

Centrifuge to separate serum or plasma from cells within 24 hours of collection. Transfer serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). A centrifuged SST tube is also acceptable at refrigeration temperature.

Preparation for Courier Transport

Transport: Serum or plasma, or centrifuged SST tube, refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Sample with recent viral loads less than 1,000 IU/mL.
  • Specimens collected in green-top heparin or white-top PPT tubes.
  • Samples not centrifuged with the serum or plasma separated within 24 hours of collection.
  • Specimens not maintained refrigerated or frozen following separation.
  • Samples exposed to repeated freeze/thaw cycles.


Once per week.
Results available within 7–10 days.

Testing is performed following HCV RNA Quantitation results.  

Reference Range

Not Applicable.

Test Methodology

Reverse Transcription Polymerase Chain Reaction (RT-PCR) of target RNA sequences and Multiplexed Hybridization Detection.


HCV genotype 1, 2, 3, 4, 5, or 6 will be reported. When HCV genotype 1 is detected, the subtype will also be reported.

Clinical Utility

The HCV genotype is of clinical importance for prognosis and options for therapeutic intervention.

Clinical Disease

Hepatitis C virus (HCV) can cause acute hepatitis. However, HCV infection presents most frequently as a chronic hepatitis diagnosed on the basis of blood liver enzyme studies. Seventy-five to eighty percent (75-80%) of patients with acute HCV develop chronic hepatitis. Chronic hepatitis C is one of the major causes of cirrhosis, and the most common indication for liver transplantation in adults in the U.S. Chronic HCV infections may also be a major cause of hepatocellular carcinoma in the world.

CPT Code


Test Codes


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