Details

Hepatitis C Virus Genotype

HCV Genotyping, HCV Typing, Hep C, HCV Mutation Analysis, HCV Genotype , EPIC: LAB6530, SOFT: IHCVG

Specimen Collection Criteria

Collect: Serum OR plasma.

  • Serum: One Gold-top SST or plain Red-top tube.
  • Plasma: One Lavender-top EDTA tube.
  • Minimum volume and pediatric minimum 1.5 mL serum or plasma.
  • Separate specimens must be submitted when multiple tests are ordered. 

Physician Office/Drawsite Specimen Preparation

Centrifuge to separate serum or plasma from cells within 24 hours of collection. Transfer serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). A centrifuged SST tube is also acceptable at refrigeration temperature.

Preparation for Courier Transport

Transport: Serum or plasma, or centrifuged SST tube, refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Sample with recent viral loads less than 1,000 IU/mL.
  • Specimens collected in green-top heparin or white-top PPT tubes.
  • Samples not centrifuged with the serum or plasma separated within 24 hours of collection.
  • Specimens not maintained refrigerated or frozen following separation.
  • Samples exposed to repeated freeze/thaw cycles.

Performed

Once per week.
Results available within 7–10 days.

Testing is performed following HCV RNA Quantitation results.  

Reference Range

Not Applicable.

Test Methodology

Reverse Transcription Polymerase Chain Reaction (RT-PCR) of target RNA sequences and Multiplexed Hybridization Detection.

Interpretation

HCV genotype 1, 2, 3, 4, 5, or 6 will be reported. When HCV genotype 1 is detected, the subtype will also be reported.

Clinical Utility

The HCV genotype is of clinical importance for prognosis and options for therapeutic intervention.

Clinical Disease

Hepatitis C virus (HCV) can cause acute hepatitis. However, HCV infection presents most frequently as a chronic hepatitis diagnosed on the basis of blood liver enzyme studies. Seventy-five to eighty percent (75-80%) of patients with acute HCV develop chronic hepatitis. Chronic hepatitis C is one of the major causes of cirrhosis, and the most common indication for liver transplantation in adults in the U.S. Chronic HCV infections may also be a major cause of hepatocellular carcinoma in the world.

CPT Code

87902

Test Codes

EPIC: LAB6530, SOFT: IHCVG

Last Updated

7/11/2019