Details

Human Immunodeficiency Virus Genotyping for Drug Resistance (HIV-1)

HIV Genotyping, HIV Resistance Testing, HIV mutation analysis , IBT #1901, EPIC: LAB6883, SOFT: XHIVG

Instructions

Note: HIV-1 Viral Load must be at least 1000 copies/mL to perform this test.

Specimen Collection Criteria

Collect: Two Lavender-top EDTA tubes.

Physician Office/Drawsite Specimen Preparation

Centrifuge to separate plasma from cells within 6 hours of collection. Transfer plasma to a plastic transport tube and freeze (-20°C/-4°F or below).

Preparation for Courier Transport

Transport: 2.0 plasma, frozen (-20°C/-4°F or below). (Min: 1.0 mL)

Rejection Criteria

  • Specimens not collected and processed as indicated.

Performed

Monday - Friday.
Results available in 5-9 days.

Reference Range

By report.

Test Methodology

Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and DNA Sequencing.

Interpretation

The recent availability of potent antiretroviral drugs has greatly improved the treatment of individuals infected with HIV. However, due to the high mutation rate of the virus, resistance to these drugs may develop, correlating with clinical progression as defined by commonly accepted endpoints, such as increased viral loads and decreased CD4+ cell counts. As drug resistance becomes more common so does the percentage of newly positive people infected with viral strains already drug resistant.

Clinical Utility

This test is useful for monitoring the drug resistance profiles in patients undergoing antiviral therapy for HIV-1 infection. This assay detects mutations in the HIV viral genes that encode the HIV protease, reverse transciptase, and integrase.

Clinical Disease

Primary HIV infections are symptomatic in 50 - 70% of patients. These patients present with a variety of symptoms including Influenza-like or mononucleosis-like illness to more severe neurological symptoms. These symptoms may persist for a few days or up to two months. Rapid progression to AIDS is seen in patients that experience a longer acute illness. Most patients however, remain asymptomatic for 1 to greater than 10 years before the clinical symptoms of AIDS present.

CPT Code

87901, 87906.

HIV-1 Viral Load testing may be required prior to HIV-1 genotyping to ensure that a minimum viral load of 1,000 copies/mL is present. 

Test Codes

IBT #1901, EPIC: LAB6883, SOFT: XHIVG

Last Updated

7/11/2019