HLA Class II Disease Association
HLA Class II Typing for Disease Association, HLA typing , tissue typing , HLA-DR, DQ typing , EPIC: LAB6424, SOFT: CIIDA
Specimen Collection Criteria
Collect: One Yellow-top ACD tube OR one Lavender-top EDTA tube.
Invert several times to mix well.
Physician Office/Drawsite Specimen Preparation
Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.
Preparation for Courier Transport
Transport: Whole blood at room temperature (20-25°C or 68-77°F).
Specimens drawn in green, clot, or SST tubes are unacceptable and will not be tested. Unlabeled specimens will be rejected.
Results typically available within 5 business days.
Polymerase Chain Reaction (PCR).
HLA Class II genotyping defines the antigens of the HLA-DRB1 and DQB1 loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-DRB1 and DQB1 alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis for disease association purposes.
EPIC: LAB6424, SOFT: CIIDA