Human Anti Mouse Antibody

HAMA, Human Anti-Mouse Antibody , ARUP #98299, EPIC: LAB6277, SOFT: XHAMA

Specimen Collection Criteria

Collect: One plain Red-top tube.

Do not use Serum Separator Tubes.

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and freeze (-20°C/-4°F or below).

Preparation for Courier Transport

Transport: 1.0 mL serum, frozen (-20°C/-4°F or below). (Min: 0.5 mL)

Rejection Criteria

  • Grossly lipemic specimens.
  • Serum Separator (SST) tubes.
  • Specimens not collected and processed as indicated.


Results available in 8-18 days.

Reference Range

By report.

Test Methodology

Quantitative Enzyme-Linked Immunosorbent Assay.

Clinical Utility

In patients receiving multiple injections of murine monoclonal IgG, immune response directed against the same IgG, may produce significant level of Human Anti-Mouse Antibodies (HAMA) in serum. Circulating levels of HAMA can bind to the injected IgG and reduce the efficacy of the antibody therapy. Also, approxmately 9% of a normal population have pre-existing HAMA reactivity without the administration of murine monoclonal IgG.

Human anti-animal antibodies cause interferences in immunological assays. The most common human anti-animal antibody interferent is HAMA. This can cause both positive and negative interferences in two-site antibody-based assays.

CPT Code


Test Codes


Last Updated