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Insulin Antibodies

Test Codes

EPIC: LAB6070, Beaker: XIA, Mayo: INAB

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One plain Red-top tube.
Also acceptable: One Gold SST tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.5 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Rejection Criteria

• Hemolyzed specimens.
• Lipemic specimens.
• Icteric Specimens
• Specimens not collected and processed as indicated.
  

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 1.5 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): 28 days
Frozen (-20°C/-4°F or below): 28 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratories in Rochester, MN.

Performed

Sunday, Wednesday
Results available in 6-10 days.

Reference Range

By report.

Test Methodology

Quantitative Radioimmunoassay (RIA).

Clinical Utility

This assay aids in the detection of insulin antibody in patients who inject insulin for a longer period of time, either human, pork, or beef insulin. Insulin-related peptides (impurities) are invariably present in most insulin preparations and are the major immunogenic components in insulin. IgG antibodies are formed to the peptides so that some of each new dose of insulin is complexed with these antibodies and is not available for its glucose-depressing functions. Patients require larger doses of insulin because of this insulin resistance. These insulin antibodies are also associated with "factitious hypoglycemia" where hypoglycemia is due to the injection of insulin rather than to a clinical problem such as insulinoma.

CPT Codes

86337

Contacts

Last Updated

10/5/2023