Lab Test

Dengue Virus Total Antibodies

Breakbone Fever

Test Codes

Mayo: DENGM, EPIC: LAB6252, SOFT: XDENG

Instructions

Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute samples. Please mark sample plainly as "acute" or "convalescent."

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 0.5 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.4 mL)

Rejection Criteria

  • Hemolyzed specimens.
  • Severely lipemic specimens.
  • Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 0.5 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.4 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): unacceptable
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratories in Rochester, MN.

Performed

Tuesday.
Results available in 2-8 days.

Reference Range

By report.

Test Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay.

Interpretation

By report.

Clinical Utility

This assay aids in the diagnosis of dengue. Dengue-specific IgG and IgM antibody concentrations rise in parallel. Serum IgM concentrations decline rapidly after 2 weeks and are undetectable after 2 months. In primary infections, specific IgM concentrations are substantially higher than IgG concentrations; whereas, in secondary infections, IgG concentrations are higher than IgM concentrations.

This is a reportable disease. All confirmed cases of dengue will be reported to the Oakland County Health Department.

CPT Codes

86790 x 2.
LOINC:  69048-7

Contacts

Last Updated

7/16/2021

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