Lab Test

Sjogren SSA Antibody

Sjogren, Anti-Ro, Ro Antibodies, SSA Antibodies, Antrim #30964, EPIC: LAB5767, SOFT: SJAA

Test Codes

Antrim #30964, EPIC: LAB5767, SOFT: SJAA

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)

Rejection Criteria

  • Plasma specimens.
  • Severely hemolyzed, lipemic or icteric specimens.

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 2 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Monday – Friday.
Results available within 2 business days.

Reference Range

Negative: Less than 100 AU/mL.
Equivocal: 100-120 AU/mL.
Positive: Greater than 120 AU/mL.

Test Methodology

Multiplex Bead Immunoassay.

Interpretation

SSA (Ro) antibodies are seen in 70-75% of Sjogren's, 30-40% of SLE and 5-10% of progressive systemic sclerosis (PSS).

The antibody to SSA (Ro) occurs in 62% of patients with "ANA negative" SLE, 63% of patients with subacute cutaneous erythematosus, 75% of the homozygous C2-deficient patients with an SLE-like picture, and in 80% of patients with Sjogren's syndrome who have vasculitis.

Pregnant SLE patients should be screened for anti-SSA antibodies. These antibodies have been closely associated with the development of neonatal congenital heart block and neonatal lupus.

Fluctuating titers of anti-SSA antibodies have been seen in some SLE patients. In these patients changes in anti-SSA levels often correlated with disease activity and anti-DNA antibody levels.

SLE patients that produce both SSA and SSB antibodies have a much lower incidence of nephritis than those patients producing anti-SSA antibodies alone.

Clinical Utility

The SSA (Ro) antibody assay is used to aid in the diagnosis and prognosis of certain rheumatic autoimmune diseases.

CPT Codes

86235

Contacts

Last Updated

10/23/2019

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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.