Sjogren SSA Antibody
Sjogren, Anti-Ro, Ro Antibodies, SSA Antibodies , Antrim #30964, EPIC: LAB5767, SOFT: SJAA
Specimen Collection Criteria
Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)
Physician Office/Drawsite Specimen Preparation
Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)
Preparation for Courier Transport
Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)
- Plasma specimens.
- Severely hemolyzed, lipemic or icteric specimens.
Centrifuged SST tubes may be transported and stored at ambient
temperatures (2o to 30o C; 36o to 86o F) for up to 48 hours.
DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum
should be removed from the gel and refrigerated (2o-8o C) or frozen
at -20o C or below.
Red-top tubes and Microtainers® may be stored at ambient temperatures
for up to 16 hours and for up to 3 days at refrigerator temperatures (2o
- 8o C). DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage,
the serum should be removed from the clot and refrigerated (2o-8o
C) or frozen at -20oC or below.
Serum specimens (pour-overs) may be stored at room temperature
(20o to 26o C; 68o to 79o F) for up to 1 week, refrigerator
temperature (2o to 8o C; 36o to 47o F) for two weeks, and
at -20o C (-4o F) for up to 3 months. Specimens stored at
-70o C (-94o F) can be stored indefinitely.
Monday – Friday.
Results available within 2 business days.
Negative: Less than 100 AU/mL.
Equivocal: 100-120 AU/mL.
Positive: Greater than 120 AU/mL.
Multiplex Bead Immunoassay.
SSA (Ro) antibodies are seen in 70-75% of Sjogren's, 30-40% of SLE and 5-10% of progressive systemic sclerosis (PSS).
The antibody to SSA (Ro) occurs in 62% of patients with "ANA negative" SLE, 63% of patients with subacute cutaneous erythematosus, 75% of the homozygous C2-deficient patients with an SLE-like picture, and in 80% of patients with Sjogren's syndrome who have vasculitis.
Pregnant SLE patients should be screened for anti-SSA antibodies. These antibodies have been closely associated with the development of neonatal congenital heart block and neonatal lupus.
Fluctuating titers of anti-SSA antibodies have been seen in some SLE patients. In these patients changes in anti-SSA levels often correlated with disease activity and anti-DNA antibody levels.
SLE patients that produce both SSA and SSB antibodies have a much lower incidence of nephritis than those patients producing anti-SSA antibodies alone.
The SSA (Ro) antibody assay is used to aid in the diagnosis and prognosis of certain rheumatic autoimmune diseases.
Antrim #30964, EPIC: LAB5767, SOFT: SJAA
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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.