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Parvovirus B19 by PCR

Test Codes

Serum: EPIC: LAB1230135, Viracor: 1510, Plasma: EPIC: LAB1230130, Viracor: 1501 CSF: EPIC: LAB1230131, Viracor: 1503, Bone Marrow: LAB1230132, Viracor: 1504 Tissue: LAB1230287, Viracor: 1505, Amniotic Fluid: EPIC: LAB901, Viracor: 1507, BAL: EPIC: LAB1230134, Viracor: 1509, Bronch Wash: EPIC: LAB1230139, Viracor: 1526, Pericardial Fluid: EPIC: LAB1230137, Viracor: 1526, Pleural Fluid: EPIC: LAB1230136, Viracor: 1511, Trach Aspirate: EPIC: LAB1230138, Viracor:1519, Trach Wash: EPIC: LAB1230140, Viracor: 1548, Spinal Fluid: EPIC: LAB1230131.

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Pink or one Lavender-top EDTA tube, or plain Red-top tube.

Also acceptable: Amniotic fluid, Cerebrospinal Fluid (CSF), Bone Marrow, Bronch Wash, Pericardial Fluid, Pleural Fluid, Trach Aspirate, Trach Wash, paraffin-embedded tissue, or fresh frozen tissue.

Specimen source is required.

Physician Office/Draw Specimen Preparation

• Centrifuge to separate plasma or serum from cells. Transfer serum or plasma to a plastic transport tube and keep at room temperature (20-26°C or 68-78.8°F).
• Maintain CSF, Bone Marrow, BAL, Bronch Wash, or Trach Aspirate specimens frozen (-20°C/-4°F or below) prior to transport.
• Maintain Pleural fluid, Amniotic Fluid, or Pericardial Fluid at room temperature (20-26°C or 68-78.8°F).
• Fresh tissue in sterile container must be frozen (-20°C/-4°F or below). Do not add water, saline or other fluid media to container. Paraffin-embedded tissue is not acceptable.

Preparation for Courier Transport

Transport: 2.0 mL serum, plasma, pericardial and pleural fluid, or amniotic fluid at room temperature (20-26°C or 68-78.8°F), Trach Aspirate, Trach Wash, Bronch Wash, BAL, Bone Marrow, CSF or fresh tissue, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL)

Rejection Criteria

• Heparinized specimens.
• Specimens in non-sterile or leaking containers.
• Specimens not collected and processed as indicated.

In-Lab Processing

• Centrifuge to separate plasma or serum from cells. Transfer serum or plasma to a plastic transport tube and keep at room temperature (20-26°C or 68-78.8°F).
  
• Maintain CSF, Bone Marrow, BAL, Bronch Wash, or Trach Aspirate specimens frozen (-20°C/-4°F or below) prior to transport.
• Maintain Pleural fluid or Pericardial Fluid at room temperature (20-26°C or 68-78.8°F).
• Fresh tissue in sterile container must be frozen (-20°C/-4°F or below)  Do not add water, saline or other fluid media to container. Paraffin-embedded tissue is not acceptable.

Transport: 2.0 mL serum, plasma, pericardial and pleural fluid, or amniotic fluid at room temperature (20-26°C or 68-78.8°F), Trach Aspirate, Trach Wash, Bronch Wash, BAL, Bone Marrow, CSF or fresh tissue, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL)

 

 

 

Storage

Specimen Stability for Testing:

Serum, Plasma, Amniotic Fluid, Pericardial and Pleural Fluid
Room Temperature (20-26°C or 68-78.8°F): 96 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 6 months

All Other Specimen Types
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable 
Frozen (-20°C/-4°F or below): 6 months

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions. 

Laboratory

Sent to Eurofins/Viracor Laboratory in Lenexa, KS.

Performed

Monday – Saturday.
Results available in 1-3 days.

Reference Range

See report.

Test Methodology

Quantitative Polymerase Chain Reaction (PCR).

Clinical Utility

Parvovirus B19 manifests itself as an acute or chronic hematological disorder in immunocompromised patients. It can cause persistent anemia, sometimes associated with leukopenia and thrombocytopenia. Pediatric transplant patients are at risk for chronic infections, which can be associated with lung and/or renal disorders. Quantitative DNA PCR can be used to detect the presence of the virus, track the course of infection, and monitor response to treatment.

CPT Codes

87799

Contacts

Last Updated

9/19/2024