Lab Test

Newborn Type with DAT

Cord Blood Evaluation, Newborn Type/Gel DAT , Newborn Type/Gel DAT: EPIC: LAB5278, SOFT: NBTD, Newborn ABO Group: EPIC: LAB5276, SOFT: NBABO, Newborn Rh Type: EPIC: LAB5277, SOFT: NBRH

Instructions

This test is for neonatal patients (less than 4 months) only.

Specimen Collection Criteria

Collect (preferred specimen): Infant Heel Stick - One Pink-top EDTA Microtainer®, properly labeled. (Minimum Volume: 0.5 mL)
Also acceptable (Farmington Hills, Grosse Pointe and Troy only): Cord Blood* - One Pink-top EDTA tube, properly labeled (single birth only). (Minimum Volume: 0.5 mL)

It is extremely important that blood samples are accurately labeled. Inpatient labels must include the following information:

Farmington Hills, Grosse Pointe, Royal Oak, Troy, Dearborn, Trenton & Wayne

Cord Blood Labeling:

  1. Patient's Name: Complete name of mother (first and last) with the words "cord blood" written on the label.
  2. Wrist Band Number of the Infant: Include the prefix letter, 5 numbers (example: N12345). Wrist band numbers are not required on cord blood samples, as they are not used for blood transfusion purposes.
  3. Hospital Identification Number: Mother's hospital ID number must be on all cord blood specimens.
  4. Date and Time Collected
  5. Identification of Collector:Employee ID number must be on the sample tube or documented in the computer system.

*Note: Cord blood specimens cannot be used for transfusion purposes. Samples cannot be tested for a Gel DAT more than 24 hours past the time of collection.

Heel Stick Labeling: 

  1. Patient's Name: Complete name, last name first. If the patient uses a first initial followed by a middle name, both must appear in proper order on the label.
  2. Wrist Band Number: Include the prefix letter, 5 numbers (example: N12345).
    NOTE: Newborns who have not yet been discharged will likely still be wearing the "N" wristband; so for example, the sample should be labeled in the format "N12345." Specimens lacking this complete information cannot be used for blood transfusion purposes and will be rejected.
  3. Hospital Identification Number: The seven digit medical record number must be included on the label.
  4. Date and Time Collected
  5. Identification of Collector: Employee ID number must be on the sample tube or documented in the computer system.

Physician Office/Drawsite Specimen Preparation

Do NOT refrigerate the specimen. Maintain specimen at room temperature (20-26°C or 68-78.8°F) prior to transport and deliver to the Laboratory immediately after collection.

Preparation for Courier Transport

Transport: Whole blood, at room temperature (20-25 °C or 68-77 °F).

Rejection Criteria

  • Cord blood samples from multiple births.
  • Improperly labeled specimens.
  • Specimens with inadequate volume.
  • Specimens collected in SST tubes.
  • Specimens contaminated with IV fluids.
  • Specimens grossly contaminated with Wharton's Jelly.
  • Hemolyzed samples will be tested only in emergency situations. 

Performed

Sunday – Saturday, 24 hours a day.
Results available within 2 hours of receipt in the Blood Bank for routine inpatient requests and within 8 hours of receipt in the Blood Bank for outpatient specimens.

Reference Range

Negative.

Test Methodology

Hemagglutination.

Interpretation

  • This test is for neonatal patients only.
  • ABO/Rh: By report.
  • The DAT detects the presence or absence of IgG on human red blood cells. It does not detect anti-C3 sensitization.

Clinical Utility

  • The ABO system is the most important blood group antigen system in transfusion therapy. ABO and Rh typing is crucial to reduce the risk of transfusion reactions in patients receiving blood and blood components.
  • The direct antiglobulin test detects in vivo sensitization of red cells. Direct antiglobulin testing is useful in the diagnosis of hemolytic disease of the newborn (HDN), auto immune hemolytic anemia, drug induced hemolytic anemia, and adverse reactions to transfusion due to RBC incompatibility.

CPT Code

86900 (ABO), 86901 (Rh), 86880 (DAT)

Test Codes

Newborn Type/Gel DAT: EPIC: LAB5278, SOFT: NBTD, Newborn ABO Group: EPIC: LAB5276, SOFT: NBABO, Newborn Rh Type: EPIC: LAB5277, SOFT: NBRH

Last Updated

7/11/2019

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UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

 

This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.