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NAT (Neonatal Alloimmune Thrombocytopenia)

NAT, NAIT, Neonatal Alloimmune Thrombocytopenia, Platelet Antibody Identification Panel

Test Codes

Blood Center of Wisconsin: BCW5603, Testing requires pathology approval prior to collection.

Department

Send Outs

Instructions

This test is not included in the Beaumont Laboratory testing formulary, however, the Laboratory can facilitate specimen collection.

• This test requires pathology review. Contact the Send Outs Laboratory at 248-551-9045 before ordering this test.
• Testing requires the ordering physician to complete forms for the independent clinical laboratory performing the testing.
• Specimens received without the appropriate forms and information will not be shipped.
• Once approved, order test as a Miscellaneous Send Out (XMISC). 

Specimen Collection Criteria

Collect: Four Yellow-top ACD (Solution A) tubes from the mother and the father, AND four plain Red-top tubes from the mother, after obtaining necessary pathology approval.

Physician Office/Draw Specimen Preparation

• Let Red-top tubes clot and centrifuge to separate serum from cells. Transfer 10 mL serum to plastic transport tubes and refrigerate (2-8°C or 36-46°F).
• Do not centrifuge Yellow-top ACD tubes. Maintain whole blood in ACD tubes refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: 30 mL whole blood from the mother and the father in ACD Solution A tubes, and 10 mL serum from the mother, refrigerated (2-8°C or 36-46°F). (Minimum: 20 mL whole blood from both the mother and the father, and 3 mL serum.)

Rejection Criteria

• Specimens received without prior Laboratory notification and review.
• Specimens not received by referral laboratory within 4 days of collection.
• Specimens not collected and processed as indicated.

In-Lab Processing

• Let Red-top tubes clot and centrifuge to separate serum from cells. Transfer 10 mL serum to plastic transport tubes and refrigerate (2-8°C or 36-46°F).
• Do not centrifuge Yellow-top ACD tubes. Maintain whole blood in ACD tubes refrigerated (2-8°C or 36-46°F) prior to transport.

Transport: 30 mL whole blood from the mother and the father in ACD Solution A tubes, and 10 mL serum from the mother, refrigerated (2-8°C or 36-46°F). (Minimum: 20 mL whole blood from both the mother and the father, and 3 mL serum.)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Blood Center of Southeastern Wisconsin, Milwaukee, WI.

Performed

Varies.
Results available in 10 days.

Reference Range

By report.

Test Methodology

Flow Cytometry, Platelet Antibody Bead Array and Polymerase Chain Reaction (PCR).

Interpretation

This assay includes: Platelet Antigen Typing of mother and father (Level 1) and Platelet Antibody Identification (Level 1).

Clinical Utility

This assay is used to detect maternal platelet-specific antibodies reactive with paternal platelets and to confirm antibody specificity by determining platelet antigen incompatibility.

CPT Codes

81105 x 2
81106 x 2
81107 x 2
81108 x 2
81109 x 2
81110 x 2
81111 x 2
81112 x 2
86022

Contacts

Last Updated

9/18/2023