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Lyme Disease Serology

Lyme Antibody (IgG/IgM), Lyme Ab, Lyme Antibody, IgG/IgM, Lyme Antibody, IgG and IgM

Test Codes

EPIC: LAB1230571, Beaker: LYME

Department

Special Chemistry

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 6.0 mL)

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 1.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 1.5 mL)

Rejection Criteria

• Plasma specimens.
• Severely lipemic, icteric, or hemolyzed specimens. 

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 1.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gel
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gel
Room Temperature (20-25°C or 68-77°F): 8 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 1 year

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Seven days a week.
Results available within 3 business days.

Reference Range

Positive, Negative.

Test Methodology

Multiplex Flow Assay

Interpretation

A negative test result indicates that both IgG and IgM are negative. A positive test result indicates that IgG, IgM, or both are positive. All positive and equivocal results are sent out for Lyme Immunoblot testing. Prompt antibiotic treatment can interfere with specific antibody responses.

Clinical Utility

This assay aids in the diagnosis of infection with B. burgdorferi.

CPT Codes

86618
LOINC:  6320-6

Contacts

Last Updated

11/4/2024