Lab Test

Lipid Panel

Antrim #13480, EPIC: LAB5084, SOFT: LIPID


It is recommended that the patient fast for 9-12 hours prior to blood draw.

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)

Contact Laboratory for acceptability of other tube types.
See Minimum Pediatric Specimen Requirements for Microtainer® collection.

Physician Office/Drawsite Specimen Preparation

Let SST specimens clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection. (Min: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged SST tube, refrigerated (2-8°C or 36-46°F). (Min: 0.5 mL)

Rejection Criteria

  • Moderate to grossly hemolyzed specimens.
  • Red-top tubes with serum not separated from cells within two hours of collection.


Centrifuged SST tubes and Microtainers® with separator gel may be transported and stored at room temperature (up to 25oC or 77oF) for 2-4 hours. DO NOT FREEZE TUBES CONTAINING WHOLE BLOOD. For longer storage, the specimen should be refrigerated (2o-8oC).

Red-top tubes and Microtainers® without separator gel may be stored at room temperature for up to 2 hours. DO NOT FREEZE TUBES CONTAINING WHOLE BLOOD. For longer storage, the serum should be removed from the clot and refrigerated (2o-8oC).

Serum specimens or (pour-overs) may be stored at room temperature (up to 25oC or 77oF) for 2-4 hours. For longer storage the sample should be refrigerated (2o to 8oC; 36o to 47o F) for one week.


Sunday - Saturday, 24 hours a day.
Results available within 4 hours.

Reference Range

See specific analytes for reference range for: Cholesterol, HDL Cholesterol, and Triglyceride.

LDL Cholesterol, Calculated

Age 0-19: 50 - 109 mg/dL.
Age 20 or Older:
50 - 129 mg/dL.

LDL Cholesterol Reference Range
Less than 70 mg/dL: Optimal for a very high risk person.
Less than 100 mg/dL: Optimal for a high risk person.
100-129 mg/dL: Near or above optimal.
130-159 mg/dL: Borderline high.
160-190 mg/dL: High.
Greater than 190 mg/dL: Very high.

LDL Cholesterol, Calculated =
 Total Cholesterol - [(HDL - Cholesterol) - (Triglycerides/5)].
Non-HDL Cholesterol, Calculated =
 Total Cholesterol - HDL Cholesterol.

Total cholesterol: HDL cholesterol ratio-risk of coronary artery disease
Less than 5.0 Desirable
5.0 - 6.0 Borderline
Greater than 6.0 High

NOTE: The appropriate LDL cholesterol level for an individual depends on overall risk factors for cardiovascular disease. According to ATP III guidelines risk factors are ASCVD (coronary heart disease, symptomatic carotic artery disease, peripheral arterial disease, abdominal aortic aneurysm), diabetes mellitus, cigarette smoking, hypertension, low HDL cholesterol (less than 40 mg/dL), family history of premature CHD and age (males 45 years or older, females 55 years or older). An HDL cholesterol 60 mg/dL or greater is regarded as a negative risk factor.

Test Methodology

Enzymatic, Spectrophotometry, Detergent Solubilization/Enzymatic.


See specific analytes for interpretations.
This panel includes: Cholesterol, HDL Cholesterol, Triglyceride, and calculations for LDL Cholesterol, Non - HDL Cholesterol and Cholesterol / HDL ratio.

NOTE: LDL Cholesterol cannot be calculated when Triglycerides are greater than 400 mg/dL.

LDL Cholesterol, Calculated =
 Total Cholesterol - [(HDL - Cholesterol) - (Triglycerides/5)].
Non-HDL Cholesterol, Calculated =
 Total Cholesterol - HDL Cholesterol.

Clinical Utility

This panel aids in the evaluation of hyperlipidemia as an index of coronary artery disease.

Several organizations have issued guidelines for management of dyslipidemias, all aiming to standardize and optimize patient care. The recent ACC/AHA guidelines aim to reduce/prevent heart disease, peripheral vascular disease and stroke by taking into account life style and lipid levels (1). Based on this information an estimate of ASCVD risk can be calculated and a decision on whether or not to treat (e.g. with statins) and modify life style can be made. The ACC/AHA guidelines do not recommend specific cholesterol set-points, but aim for a particular percent decrease in LDL cholesterol. Our lab will continue to use the ATP guideline cut-points in lipid reporting (2). The National Lipid Association also has recommendations that are similar to the ATP III guidelines (3).


  1. Stone NJ, Robinson JG, Lichetenstein AH et al: ACC/AHA guideline on the treatment of blood cholesterol to reduce atherosclerotic cardiovascular risk in adults. A report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. J Am Coll Cardiol 2014 63:2889-2934.
  2. Third report of the National Cholesterol Education Program (NCEP) Expert Panel on detection, evaluation and treatment of high blood cholesterol in adults (ATP III). Circulation 20002;106:3143-3421.
  3. Jacobson TA, Ito MK, Maki KC et al. National Lipid Association recommendations for patient-centered management of dyslipidemia. Part 1 – executive summary. J Clin Lipidol 2014;8:473-488.

CPT Code


Test Codes

Antrim #13480, EPIC: LAB5084, SOFT: LIPID

Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.