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Insulin, 2-Hour Post 75 g Glucola

Test Codes

EPIC: LAB5812, Beaker: IN2PG

Department

Chemistry

Instructions

• This test is for a single blood draw, 2 hours following the ingestion of Glucola. It is not part of a Glucose Tolerance Test (GTT) or Insulin Tolerance Test.
• The patient should fast overnight prior to specimen collection.

Specimen Collection Criteria

Collect: One Gold-top SST tube 2 hours after the ingestion of the 75 g Glucola load. (Minimum Whole Blood: 2.0 mL)

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transfer serum to a plastic aliquot tube and refrigerate (2-8°C or 36-46°F) immediately. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Serum aliquot tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)

Rejection Criteria

• Lipemic, icteric, or grossly hemolyzed specimens.
• Samples left on the clot at room temperature.
• Samples subjected to repeated freeze/thaw cycles. 

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transfer serum to a plastic aliquot tube and refrigerate (2-8°C or 36-46°F) immediately. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Automated Chemistry Laboratory

Performed

Monday – Friday.
Results available in two business days.

Reference Range

2-Hour Post 75 Gram Glucola: 29.0-93.0 microIU/mL.

Test Methodology

Chemiluminescence Immunoassay.

Interpretation

Some investigators have found the glucose to insulin ratio, 2 hours following 75 grams Glucola administration, to be a useful adjunct to screening for polycystic ovary syndrome in some patients. The results are usually used in conjunction with a fasting glucose: insulin ratio.

Patients undergoing insulin therapy form anti-insulin antibodies that are capable of interfering with this assay.

Clinical Utility

Evaluation of glucose intolerance and investigation of polycystic ovary syndrome.

Reference

1. Legro RS, Finegood D, Dunaif A. A fasting glucose to insulin ratio is a useful measure of insulin sensitivity in women with polycystic ovary syndrome. J.Clin Endocrino Metab. 83:2694-98, 1998.

CPT Codes

83525

Contacts

Last Updated

2/27/2024