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HLA Class II Typing (Southeast Michigan Only)

DNA-based histocompatibility testing, disease association, histocompatibility, HLA DR testing, Donor HLA typing, Class II Typing

Test Codes

EPIC: LAB1230935, SOFT: CIITY

Department

HLA

Specimen Collection Criteria

Collect: Two Lavender-top EDTA tubes OR one Pink-top OR one Yellow-top ACD tubes.

Invert several times to mix well.

Physician Office/Draw Specimen Preparation

Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 72 hours of collection.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F).

Rejection Criteria

• Unlabeled specimens.
• Specimens drawn in clot, SST, or Sodium Heparin tubes. 

In-Lab Processing

Maintain specimen at room temperature (20-26°C or 68-78.8°F) until testing.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Frozen (-80°C): 2 years (DNA)

Laboratory

Royal Oak HLA Laboratory

Performed

As needed.
Results typically available within 5 business days.

Test Methodology

Polymerase Chain Reaction (PCR).

Interpretation

By report.

Clinical Utility

HLA Class II genotyping defines the antigens of the HLA-DRB1 and DQB1 loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-DRB1 and DQB1 alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.

CPT Codes

81376

Contacts

Last Updated

10/31/2024