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HLA Class I Disease Association (Southeast Michigan Only)

HLA typing, HLA-A, B, C typing, tissue typing, HLA Class I Typing for disease association, HLA-A29 Genotyping (Birdshot Chorioretinopathy), HLA-B27 Genotyping (Spondyloarthropathies), HLA-B51 Genotyping (Bechet's Disease)

Test Codes

EPIC: LAB1230930, SOFT: CIDA, EPIC: HLA-A29 LAB1230936, EPIC: HLA-B27 LAB1230937, EPIC: HLA-B51 LAB1230938

Department

HLA

Specimen Collection Criteria

Collect: One Yellow-top ACD tube OR one Pink-top OR two Lavender-top EDTA tube.

Invert several times to mix well.

Physician Office/Draw Specimen Preparation

Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 72 hours of collection.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F).

Rejection Criteria

• Unlabeled specimens.
• Specimens drawn in clot, SST, or Sodium Heparin tubes.

In-Lab Processing

Maintain specimen at room temperature (20-26°C or 68-78.8°F) until testing.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Frozen (-80°C): 2 years (DNA)

Laboratory

Royal Oak HLA Laboratory

Performed

As needed.
Results typically available within 5 business days.

Test Methodology

Polymerase Chain Reaction (PCR).

Interpretation

By report.

Clinical Utility

HLA Class I genotyping defines the antigens of the HLA-A, B and C loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-A, B and C alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.

CPT Codes

81373, 81372.

Contacts

Last Updated

10/31/2024