High Sensitivity Troponin
high sensitivity Troponin I, hsTnI, troponin, troponin panel, cardiac troponin, cTnI
Test Codes
EPIC: LAB8578 High Sensitivity Troponin I (standalone); LAB8579 High Sensitivity Troponin I Baseline with Reflex to 2-hour HS Troponin
Department
Chemistry
Specimen Collection Criteria
One Pearl-top K2EDTA PPT plasma gel separator tube (BD 362788) or lavender K2EDTA plasma tube. (Minimum Whole Blood: 4.0 mL)
Do NOT use any other blood tube or microtainer.
Physician Office/Draw Specimen Preparation
Outpatient sites use only LAB8578 High Sensitivity Troponin I (standalone). Invert tube 8 times for proper mixing of EDTA anticoagulant, then centrifuge immediately to separate plasma from cells, 1300 x g for 10 minutes (e.g., Drucker horizon mini E centrifuge). Refrigerate (2-8°C or 36-46°F) the centrifuged tube. (Minimum: 1.0 mL plasma)
Preparation for Courier Transport
- Centrifuged Pearl-top or lavender-top tube, refrigerated (28°C or 36-46°F). (Minimum: 1.0 mL plasma)
- Labeled aliquot tube, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL plasma)
Rejection Criteria
- Pearl-top or lavender top tubes with plasma not separated from cells within eight hours of collection.
- Specimens collected in inappropriate collection tubes.
- Specimens collected in microtainer tubes.
- Specimens not collected and processed as indicated.
- Grossly hemolyzed specimens.
In-Lab Processing
Centrifuge Pearl-top or lavender top tubes received from inpatient locations to separate plasma from cells. For STAT troponin orders, centrifuge at 4000 x g for 3 minutes (e.g., StatSpin Express 4 centrifuge). For routine analysis, centrifuge at 1300 x g for 5 minutes. Specimens should be free of particulate matter. Deliver immediately to the appropriate testing station or add aliquoted labeled plasma sample to the automated line.
Storage
Specimen Stability for Testing:
Pearl-top K2EDTA plasma gel separator tube
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): Unacceptable
Lavender K2EDTA tube (Centrifuged and NOT Centrifuged)
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 8 hours
Frozen (-20°C/-4°F or below): Unacceptable
Laboratory
Dearborn Chemistry Laboratory
Farmington Hills Chemistry Laboratory
Grosse Pointe Chemistry Laboratory
Livonia Main Laboratory
Lenox Main Laboratory
Royal Oak Automated Chemistry Laboratory
Troy Chemistry
Laboratory
Reference Range
< 4 ng/L for single-Troponin rule-out algorithm.
Female: ≤ 17 ng/L (≤ 99th Percentile)
Male: ≤ 35 ng/L (≤ 99th Percentile)
See attached algorithm for significant 2-hour delta Troponin values.
Test Methodology
Automated Chemiluminescence Immunoassay.
Clinical Utility
> or = 200 ng/L
Delta Troponin > or = 10 ng/L, positive or negative change
CPT Codes
84484.
Contacts
Chemistry Laboratory – DBN
313-436-2196
Name: Chemistry Laboratory – DBN
Location:
Phone: 313-436-2196
Chemistry Laboratory – FH
947-521-5252
Name: Chemistry Laboratory – FH
Location:
Phone: 947-521-5252
Chemistry Laboratory – GP
313-473-1807
Name: Chemistry Laboratory – GP
Location:
Phone: 313-473-1807
Lenox Laboratory – LNX
947-523-4070
Name: Lenox Laboratory – LNX
Location:
Phone: 947-523-4070
Livonia Laboratory – LVA
947-523-4370
Name: Livonia Laboratory – LVA
Location:
Phone: 947-523-4370
Chemistry Laboratory – RO
248-551-8068
Name: Chemistry Laboratory – RO
Location:
Phone: 248-551-8068
Chemistry Laboratory – TR
248-964-8070
Name: Chemistry Laboratory – TR
Location:
Phone: 248-964-8070
Last Updated
1/19/2024
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