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Haemophilus Influenzae B IgG

Haemophilus Influenzae Type b Antibody, IgG

Test Codes

EPIC: LAB6045, Beaker: XHAIG, Mayo: HIBSG

Department

Send Outs

Instructions

"Pre" and "post" Haemophilus influenzae B vaccination samples should be submitted together for testing. "Post" samples should be drawn 30 days after immunization and must be received within 60 days of "pre" sample. Please clearly mark samples "pre-vaccine" or "post-vaccine" so that samples will be saved and tested simultaneously.

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 0.5 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.4 mL)

Rejection Criteria

• Hemolyzed specimens.
• Severely lipemic specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.15 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 7 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Laboratories, Rochester, MN.

Performed

Monday, Friday.
Results available in 2-4 days.

Reference Range

Less than 0.15 mcg/mL: Antibody concentration not protective.

Greater than or equal to 0.15 mcg/mL: Antibody to Haemophilus influenzae B detected. Suggestive of protection.

Test Methodology

Enzyme Immunoassay.

Interpretation

Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza B antibody, IgG as follows:

1. If the post-vaccination concentration is less than 3.0 mcg/mL, the patient is considered to be a non-responder.
2. If the post-vaccination concentration is greater than or equal to 3.0 mcg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a non-responder.

Clinical Utility

This assay is used to assess the production of antibody against a protein-polysaccharide conjugate vaccine.

CPT Codes

86684

Contacts

Last Updated

10/10/2023