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Glucose Phosphate Isomerase, Erythrocytes

Test Codes

EPIC: LAB6039, Beaker: XGPI, Mayo: GPI1

Department

Send Outs

Specimen Collection Criteria

Collect: One Yellow-top ACD (Solution B) tube.
Also acceptable: Two Lavender-top EDTA tubes.

Send specimen for processing immediately after collection. 

Physician Office/Draw Specimen Preparation

Do not centrifuge or freeze. Maintain whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F), prior to transport.

Preparation for Courier Transport

Transport: 6.0 mL whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Rejection Criteria

• Centrifuged specimens.
• Frozen specimens.
• Grossly hemolyzed specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge or freeze. Maintain whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F), prior to transport.

Transport: 6.0 whole blood in original collection tubes, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 20 days
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Medical Laboratories, Rochester, MN.

Performed

Tuesday, Thursday.
Results available in 1-6 days.

Reference Range

By report.

Test Methodology

Kinetic Spectrophotometry.

Interpretation

By report.

Clinical Utility

This assay is used as a second order test in the evaluation of individuals with chronic hemolysis. Erythrocyte glucose phosphate isomerase (GPI) deficiency is a common cause of chronic hemolysis. GPI is an autosomal recessive trait. Hemolytic disease of the newborn is a common presenting manifestation of GPI deficiency.

CPT Codes

84087

Contacts

Last Updated

10/2/2023