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Diazepam Level and Metabolite

Valium

Test Codes

EPIC: LAB6004, Beaker: XDIAZ, Warde: DIAZE

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One plain Red-top tube.

Do not use Serum Separator Tubes.
Time of Collection: Trough, just prior to the next dose.

Physician Office/Draw Specimen Preparation

Centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.2 mL)

Rejection Criteria

Serum Separator (SST) tubes.

Specimens not collected and processed as indicated.

In-Lab Processing

Centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.2 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Warde Medical Laboratory, Ann Arbor, MI.

Performed

Monday, Wednesday, Friday.
Results are available with 2-5 days.

Reference Range

Diazepam: 100-1,000 ng/mL.
Nordiazepam: 100-1,000 ng/mL.
Toxic: Greater than 5,000 ng/mL.

Test Methodology

Quantitative Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Interpretation

By report.

Clinical Utility

Diazepam (Valium®) is widely used as an anti-anxiety, hypnotic, and muscle relaxant agent. The Diazepam (Valium®) assay is used to monitor therapeutic drug levels and to evaluate toxicity.

CPT Codes

80346, (Alt G0480).
LOINC: Diazepam-16195-0, 3548-5

Contacts

Last Updated

10/2/2023