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Collagen Binding Activity (CBA) Profile

CBA, CBA Profile, Collagen-Binding Activity, vWF Collagen Binding Activity, von Willebrand Factor Collagen Binding Activity

Test Codes

Mayo: ZW57, Esoterix # 500360, EPIC: LAB6242, Beaker: XCBA

Department

Send Outs

Instructions

This profile includes: von Willebrand Factor Collagen Binding Activity, von Willebrand Antigen, and Collagen Binding Ratio.

Specimen Collection Criteria

Collect: Two Light Blue-top Sodium Citrate tubes.

TUBES MUST BE FULL.

Physician Office/Draw Specimen Preparation

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 4 hours of collection. If transport within 4 hours is not possible, the following procedure must be followed for accurate results:

1. Centrifuge the capped tubes at 1500 x g for 15 minutes.
2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
3. Immediately remove only the top two-thirds of the PPP and transfer into a plastic tube.
4. Freeze (-20°C/-4°F or below) the PPP immediately.
5. Transport frozen on DRY ICE. Specimen must remain frozen during transport.  

Preparation for Courier Transport

Transport: 1.0 mL PPP, frozen (-20°C/-4°F or below). (Minimum: 0.8 mL)

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 4 hours of collection. If transport within 4 hours is not possible, the following procedure must be followed for accurate results:

1. Centrifuge the capped tubes at 1500 x g for 15 minutes.
2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
3. Immediately remove only the top two-thirds of the PPP and transfer into a plastic tube.
4. Freeze (-20°C/-4°F or below) the PPP immediately.
5. Transport frozen on DRY ICE. Specimen must remain frozen during transport. 

Transport: 1.0 mL PPP, frozen (-20°C/-4°F or below). (Minimum: 0.8 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 4 hours
Frozen (-20°C/-4°F or below): 60 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratories in Rochester, MN. Forwarded to Esoterix Colorado Coagulation Laboratory.

Performed

Saturday.
Results available in 3-8 days.

Reference Range

By report.

Test Methodology

Enzyme-Linked Immunosorbent Assay (ELISA), Calculation, LIA.

Clinical Utility

The collagen binding assay (CBA) can aid in the differentiation between von Willebrand disease type I and II when used in conjunction with the vWF antigen assay. This assay evaluates the ability of high molecular weight multimers present in the patient's sample to bind collagen which is coated on the microtiter plates.

CPT Codes

83520, 85246.

Contacts

Last Updated

8/15/2023