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Blastomyces Antibody by Immunoassay with Reflex to Immunodiffusion

Test Codes

EPIC: LAB1231263, Beaker: XBLAS, ARUP #3000236

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.30 mL)

Rejection Criteria

• Contaminated Specimen.
• Hemolyzed Specimen.
• Severe Lipemia.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.30 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 1 year

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to ARUP Laboratories, Salt Lake City, UT.

Performed

Saturday – Sunday
Results available 3-6 days.

Reference Range

None detected.

Test Methodology

Semi-Quant Enzyme-Linked Immunosorbent Assay and Qualitative Immunodiffusion.

Interpretation

A positive result may suggest active or recent infection. The test is positive in about 80% of cases. Cross reactions occur, especially with histoplasmosis. A negative test (none detected) does not exclude blastomycosis.

NOTE: This test uses a partially purified "A" antigen of Blastomyces dermatitides.

Clinical Utility

This assay is used to aid in the diagnosis of Blastomyces dermatitidis infections.

CPT Codes

If reflexed 86612.
LOINC:  EIA 7816-2, Precipitin 5058-3

Contacts

Last Updated

10/8/2024