Lab Test

Bilirubin in Amniotic Fluid

Amniotic Fluid Analysis for Hemolytic Disease of the Newborn, Amniotic Fluid Spectural Analysis, Liley Test , ARUP #80276. Order as a Miscellaneous Sendout.

Specimen Collection Criteria

Collect: 3.0 mL amniotic fluid in a sterile collection container. (Min: 2.0 mL)

Protect specimen from light after collection. 

Physician Office/Drawsite Specimen Preparation

Protect specimen from light. Maintain specimen refrigerated (2-8°C or 36-46°F) until transport.

Preparation for Courier Transport

Transport: Light-protected amniotic fluid in a sterile collection container, refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Specimens not protected from light.
  • Specimens not collected and processed as indicated.


Sunday - Saturday.
Results available in 2-4 days.

Reference Range

Reference values are dependent on duration of pregnancy.

Test Methodology

Quantitative Spectrophotometry.


Results are reported as "corrected absorbance at 450 nm" together with the corresponding zone (lower, mid, upper) from the Liley chart. Values falling in the upper zone are usually associated with severly-affected fetuses/neonates, those in the lower zone are usually associated with unaffected or mildly affected fetuses/neonates.

Clinical Utility

Maternal RBC antibodies may cause hemolytic disease in the fetus and neonate. The level of bilirubin in amniotic fluid is proportional to the degree of hemolysis and is therefore used to assess the developing fetus.

CPT Code


Test Codes

ARUP #80276. Order as a Miscellaneous Sendout.

Last Updated


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