Lab Test

Argatroban (Dilute Thrombin TIme)

EPIC: LAB6884, SOFT: ARGAT

Test Codes

EPIC: LAB6884, SOFT: ARGAT

Instructions

Specimens with hematocrit values greater than 55% must have anticoagulant adjusted. Specimens must be recollected using a specified whole blood to sodium citrate ratio. Contact the performing Laboratory for specific instructions.

Specimen Collection Criteria

Collect: One Light Blue-top Becton-Dickinson 3.2% Sodium Citrate tube.

TUBE MUST BE FULL.

Physician Office/Draw Specimen Preparation

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 4 hours of collection. If transport within 4 hours is not possible, the following procedure must be followed for accurate results:

  1. Centrifuge the capped tube at 1500 x g for 15 minutes.
  2. Transfer plasma with a plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
  3. Immediately remove only the top two-thirds of the PPP and transfer it into a plastic tube.
  4. Freeze (-20°C/-4°F or below) the PPP immediately.
  5. Transport frozen on DRY ICE. Specimen must remain frozen during transport.   

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F), or plasma obtained through the process listed above, frozen (-20°C/-4°F or below).

Rejection Criteria

  • Glass tubes and 3.8% Sodium Citrate tubes.
  • Specimens that are clotted or grossly hemolyzed.
  • Inappropriate specimen volume.
  • Thawed or partially thawed specimens.

In-Lab Processing

Whole blood specimens must be processed as follows upon receipt in the Laboratory:

  1. Centrifuge the capped tube at 1500 x g for 15 minutes.
  2. Transfer plasma with a plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
  3. Immediately remove only the top two-thirds of the PPP and transfer it into a plastic tube.
  4. Test specimen immediately. If testing is not possible upon receipt, freeze the PPP.

For more information on this test please see procedures at 

Storage

Specimen Stability for Testing:

Whole Blood
Room Temperature (20-26°C or 68-78.8°F): 4 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Plasma Aliquot
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Frozen (-20°C/-4°F or below): 30 days

Laboratory

Royal Oak Coagulation Laboratory.

Performed

Monday – Friday, 7:00 am – 3:30 pm.
Results available within 24 hours. 

Reference Range

Therapeutic Range: 0.6-1.8 mcg/mL.

Test Methodology

Clotting Assay (IL-ACL TOP).

Interpretation

The dilute thrombin time should not be affected by warfarin or lupus anticoagulant.

Clinical Utility

The dilute thrombin time is a clotting test that can be used for quantitative measurement of argatroban in plasma. Argatroban is typically monitored using the aPTT. However, in some cases, a patient's baseline aPTT may be abnormal due to lupus anticoagulant or other coagulapathies, making monitoring of argatroban difficult. In these cases, argatroban levels can provide a more accurate assessment of drug levels.

CPT Codes

80299

Contacts

Last Updated

10/23/2019

Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

 

This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.