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Smooth Muscle Antibody, IgG Titer

ASMA, Anti-Smooth Muscle Antibody

Test Codes

EPIC: LAB512, SOFT: ASMMA, Antrim #30190

Department

Special Chemistry

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 0.5 mL)

Rejection Criteria

• Plasma specimens.
• Severely lipemic, icteric or hemolyzed specimens.

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Monday, Wednesday, Friday.
Results available within 2 business days.

Reference Range

Less than 1:20.

Test Methodology

Indirect Immunofluorescent Assay (IFA).

Interpretation

• Anti-Smooth Muscle Antibody (ASMA) is present in high titers in 70% of patients with autoimmune hepatitis. A high titer result may also be seen in primary biliary cirrhosis and primary sclerosing cholangitis. Low ASMA titers may be present in viral infections, malignancies and normal patients. ASMA usually does not appear in SLE.
• Titers in autoimmune hepatitis may reach 1:640. However, they generally range from 1:80 to 1:320 and persist for years. In viral hepatitis the titers are generally below 1:80 and are transient. Low titer positive results may occur in healthy individuals (less than 5%).

Clinical Utility

Anti-Smooth Muscle Antibody (ASMA) assay is used to aid in the diagnosis of patients with autoimmune hepatitis.

CPT Codes

86256
LOINC: 5358-7

Contacts

Last Updated

10/16/2024