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Anti-Mitochondrial Antibody

AMA, Mitochondrial Autoantibodies, Mitochondrial IgG, Anti-Mitochondrial Antibody

Test Codes

EPIC: LAB513, SOFT: AMA, Antrim #30055

Department

Immunology

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)

Rejection Criteria

• Plasma specimens.
• Severely lipemic, icteric, or hemolyzed specimens. 

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Chemistry Laboratory

Performed

Monday, Wednesday, Friday.
Results available within 2 business days.

Reference Range

Less than 1:20.

Test Methodology

Indirect Fluorescent Assay (IFA).

Interpretation

Titers in patients with primary biliary cirrhosis (PBC) range from 1:20 to 1:1280 with about 50% of PBC patients having titers between 1:200 and 1:1280.

Low anti-mitochondrial antibody (AMA) titers may be detected in various liver disorders such as chronic active hepatitis and cryptogenic cirrhosis. High AMA titers support the diagnosis of primary biliary cirrhosis. However, AMA titers do not appear to have any correlation with clinical activity since titers do not vary with the severity or progression of disease. Therefore, mitochondrial autoantibodies cannot be used to monitor responses to therapy or provide prognostic information.

Low titer positive results may occur in healthy individuals.

Clinical Utility

The anti-mitochondrial antibody assay aids in the diagnosis of primary biliary cirrhosis.

CPT Codes

86256
LOINC: 5247-2

Contacts

Last Updated

10/16/2024