Amikacin Trough Blood Level
Amikin
Test Codes
LEVEL: Antrim: 17246, EPIC: LAB1231308, Beaker: AMIKA, PEAK: Antrim: 19173, EPIC: LAB1231309, Beaker: AMIKP, TROUGH: Antrim: 19172, EPIC: LAB1231310, Beaker: AMIKT
Department
Chemistry
Specimen Collection Criteria
Collect: One plain Red-top tube. (Minimum Whole Blood: 2.0 mL)
Do not use Serum Separator Tubes.
Time of Collection: Consult Pharmacy Dosing Service*
Record the exact time of specimen collection on the tube or in the computer system.
*A common sampling time for peak measurement is 30 minutes after cessation of an IV infusion. A common sampling time for trough measurement is just prior to the next dosage administration.
Physician Office/Draw Specimen Preparation
Let specimens clot 30-60 minutes. Refrigerate (2-8°C or 36-46°F) the collection tube within two hours of collection. (Minimum: 2.0 mL blood)
Preparation for Courier Transport
Transport: Collection tube, refrigerated (2-8°C or 36-46°F). (Minimum: 2.0 mL blood)
Rejection Criteria
- Serum Separator (SST) tubes, Sodium Citrate tubes, EDTA tubes, or Oxalate/Fluoride tubes.
- Severly lipemic or hemolyzed specimens.
In-Lab Processing
Let specimens clot 30-60 minutes. Centrifuge specimen to separate serum from cells. Deliver an aliquot of serum or the collection tube immediately to the appropriate testing station.
Storage
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days
Laboratory
Royal Oak Chemistry Laboratory
Performed
Sunday – Saturday, 24 hours a day.
Results available within 24 hours.
Reference Range
- Trough Levels
Optimal: 4.0-8.0 mcg/mL.
Toxic: Greater than 10 mcg/mL.
- Peak Levels
Optimal: 20.0-30.0 mcg/mL.
Toxic: Greater than 35 mcg/mL.
Test Methodology
Particle Enhanced Turbidimetric Inhibition Immunoassay.
Interpretation
Aminoglycoside antibiotics have been demonstrated to produce nephro- and oto-toxicity. Values above targets for peaks and troughs may require dosage adjustment either by decreasing the dose or increasing the dosing interval.
Clinical Utility
Amikacin is a semisynthetic aminoglycoside antibiotic with a broad spectrum of activity against gram-negative bacteria. This assay is used to monitor the therapeutic drug level and evaluate the toxicity of amikacin.
CPT Codes
80150, 35669-1, 3319-1, 3321-7
LOINC: 12-5
Contacts
Chemistry Laboratory – RO
248-551-8068
Name: Chemistry Laboratory – RO
Location:
Phone: 248-551-8068
Last Updated
10/15/2024
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