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Beaumont Laboratory

Hepatitis C Virus Genotype

HCV Genotyping, HCV Typing, Hep C, HCV Mutation Analysis, HCV Genotype, EPIC: LAB6530, SOFT: IHCVG

Specimen Collection Criteria

Collect: Serum OR plasma.

  • Serum: One Gold-top SST or plain Red-top tube.
  • Plasma: One Lavender-top EDTA tube.
  • Minimum volume and pediatric minimum (single test with no repeat): 1.5 mL serum or plasma.
  • Separate specimens must be submitted when multiple tests are ordered. 

Physician Office/Drawsite Specimen Preparation

Centrifuge to separate serum or plasma from cells within 24 hours of collection. Transfer serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). A centrifuged SST tube is also acceptable at refrigeration temperature.

Preparation for Courier Transport

Transport: Serum or plasma, or centrifuged SST tube, refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Sample with recent viral loads less than 1,000 IU/mL.
  • Specimens collected in green-top heparin or white-top PPT tubes.
  • Samples not centrifuged with the serum or plasma separated within 24 hours of collection.
  • Specimens not maintained refrigerated or frozen following separation.
  • Samples exposed to repeated freeze/thaw cycles.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Frozen (-20°C/-4°F or below): 1 month


Royal Oak Clinical Molecular Pathology Laboratory.


Once per week.
Results available within 7 - 10 days.

Reference Range

Not Applicable.

Test Methodology

Reverse Transcription Polymerase Chain Reaction (RT-PCR) of target RNA sequences and Multiplexed Hybridization Detection.


HCV genotype 1, 2, 3, 4, 5, or 6 will be reported. When HCV genotype 1 is detected, the subtype will also be reported.

Clinical Utility

The HCV genotype is of clinical importance for prognosis and options for therapeutic intervention.

Clinical Disease

Hepatitis C virus (HCV) can cause acute hepatitis. However, HCV infection presents most frequently as a chronic hepatitis diagnosed on the basis of blood liver enzyme studies. Seventy-five to eighty percent (75-80%) of patients with acute HCV develop chronic hepatitis. Chronic hepatitis C is one of the major causes of cirrhosis, and the most common indication for liver transplantation in adults in the U.S. Chronic HCV infections may also be a major cause of hepatocellular carcinoma in the world.

Incubation Period

The incubation period is approximately 50 days (range, 15 - 150 days).


Hepatitis C virus (HCV) causes 70 - 95% of post-transfusion hepatitis infections making it the most common cause of post-transfusion hepatitis. However, with improved universal donor testing HCV infections are rare after blood transfusions. Parenteral exposure due to illicit drug use and needle sharing constitutes the major means of transmission in the U.S. HCV has also been implicated to be the cause of 65 - 90% of cases of sporadic acute viral hepatitis.

CPT Code


Test Codes


Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.