Hepatitis C Virus Genotype
HCV Genotyping, HCV Typing, Hep C, HCV Mutation Analysis, HCV Genotype, EPIC: LAB6530, SOFT: IHCVG
Specimen Collection Criteria
Collect: Serum OR plasma.
- Serum: One Gold-top SST or plain Red-top tube.
- Plasma: One Lavender-top EDTA tube.
- Minimum volume and pediatric minimum (single test with no repeat): 1.5 mL serum or plasma.
- Separate specimens must be submitted when multiple tests are ordered.
Physician Office/Drawsite Specimen Preparation
Centrifuge to separate serum or plasma from cells within 24 hours of collection. Transfer serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). A centrifuged SST tube is also acceptable at refrigeration temperature.
Preparation for Courier Transport
Transport: Serum or plasma, or centrifuged SST tube, refrigerated (2-8°C or 36-46°F).
- Sample with recent viral loads less than 1,000 IU/mL.
- Specimens collected in green-top heparin or white-top PPT tubes.
- Samples not centrifuged with the serum or plasma separated within 24 hours of collection.
- Specimens not maintained refrigerated or frozen following separation.
- Samples exposed to repeated freeze/thaw cycles.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): 3 months
Specimen Storage in Department Prior to Disposal:
Frozen (-20°C/-4°F or below): 1 month
Royal Oak Clinical Molecular Pathology Laboratory.
Once per week.
Results available within 7 - 10 days.
Reverse Transcription Polymerase Chain Reaction (RT-PCR) of target RNA sequences and Multiplexed Hybridization Detection.
HCV genotype 1, 2, 3, 4, 5, or 6 will be reported. When HCV genotype 1 is detected, the subtype will also be reported.
The HCV genotype is of clinical importance for prognosis and options for therapeutic intervention.
Hepatitis C virus (HCV) can cause acute hepatitis. However, HCV infection presents most frequently as a chronic hepatitis diagnosed on the basis of blood liver enzyme studies. Seventy-five to eighty percent (75-80%) of patients with acute HCV develop chronic hepatitis. Chronic hepatitis C is one of the major causes of cirrhosis, and the most common indication for liver transplantation in adults in the U.S. Chronic HCV infections may also be a major cause of hepatocellular carcinoma in the world.
The incubation period is approximately 50 days (range, 15 - 150 days).
Hepatitis C virus (HCV) causes 70 - 95% of post-transfusion hepatitis infections making it the most common cause of post-transfusion hepatitis. However, with improved universal donor testing HCV infections are rare after blood transfusions. Parenteral exposure due to illicit drug use and needle sharing constitutes the major means of transmission in the U.S. HCV has also been implicated to be the cause of 65 - 90% of cases of sporadic acute viral hepatitis.
EPIC: LAB6530, SOFT: IHCVG