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Beaumont Laboratory

Total Complement (CH50)

CH50, Complement CH50, Total, Antrim #30240, EPIC: LAB7024, SOFT: CH50G

Specimen Collection Criteria

Collect: One plain Red-top tube.

Critical Frozen. Document the collection time on either the tube or requisition form and send specimen immediately for processing. 

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes at room temperature (20-26°C or 68-78.8°F) then immediately centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and freeze (-20°C/-4°F or below) immediately.

Preparation for Courier Transport

Transport: Serum in a plastic transport tube, frozen (-20°C/-4°F or below). (Min: 0.5 mL)

Rejection Criteria

  • Specimens not collected and processed as indicated.
  • Specimens not received frozen from off-site areas/offices.
  • Hemolyzed or lipemic specimens


Specimen Stability for Testing:

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Frozen (-20°C/-4°F or below): 7 days


Royal Oak Special Testing Laboratory.


Tuesday, Thursday.
Results available within 3 business days.

Reference Range

Greater than or equal to 41.7 U/mL.

Test Methodology

Liposome Immunoassay (The Binding Site SPAPLUS Analyzer)

Clinical Utility

Total Complement (CH50) is a functional assay that measures the activity of complement components C1 to C9 (i.e., the classical and terminal pathways). It does not measure the functional activity of the alternative pathway. If the Total Complement (CH50) measurement is zero or very low it is advisable to repeat the test to confirm the result. If the result is confirmed, the clinical presentation should be considered prior to further testing. If the patient exhibits recurrent neisserial infection, immunochemical determination of C5, C6, C7 and C8 is advised (or a functional assay for C5-C8). If the clinical problem is angioedema, initial studies should include C3, C4, C1 inhibitor (and possibly C1q and C2). Acquired disorders such as immune-mediated glomerulonephritis and other autoimmune disorders should direct testing towards C3 and C4.

Note: In order to assess the alternative complement pathway, the alternative pathway CH50  may be ordered (send-out test).

CPT Code


Test Codes

Antrim #30240, EPIC: LAB7024, SOFT: CH50G

Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.