Specimen Collection Manual: Tissue or Wound/Lesion, Fluid Collection - Bacterial, Fungal, AFB
- Sterile non-bacteriostatic saline.
- Sterile 10 cc syringes.
- Sterile screw-capped collection containers.
- ESwab Collection System.
Wound/Lesion (Skin, Sub-Cutaneous) Site Preparation Instructions
- Decontaminate the site with surgical soap and 70% ethyl or isopropyl alcohol.
- Rinse the wound well with sterile, non-bacteriostatic saline and allow to dry.
Specimen Collection Criteria
- Wearing a sterile glove, separate the wound margins with the thumb and forefinger of one hand or to make a small opening in a closed abscess with a sterile scalpel blade.
- Extend the tip of the swab into the depths of the wound/lesion.
- Place the swab into transport media and send to the Laboratory immediately. The specimen is stable at room temperature (20-26°C or 68-78.8°F) for up to 8 hours.
- Alternately, fluid may be aspirated from a wound with a sterile syringe. That specimen can be placed into a sterile, screw-capped collection container for transport to the Laboratory.
- Collect tissues and fluids aseptically taking care to prevent cross-contamination when specimens are taken from multiple sites. Use different tubes for specimens from different sites.
- Autopsy specimens should be collected within 24 hours of the time of death.
Minimum Specimen Requirements*
|Type of Culture
||Sterile Fluid (Not CSF)
||2-4 mL (AFB requires another 5 mL)
- Do not submit tissues for culture in formalin, or in containers that ever contained formalin.
- Do not use expired media. Check expiration date on all media prior to use.
- Swab specimens are unacceptable for AFB Cultures.
|If the Specimen is for:
||Small tissue specimens should be placed into an ESwab container. Large specimens must be submitted in a sterile collection container.
||Fluid specimens must be submitted in a sterile collection container. Do NOT add saline with fluid specimens. Do NOT submit swab specimens.
||Place ESwab in an appropriate transport medium.
- Send specimens to the Laboratory at room temperature (20-26°C or 68-78.8°F). Do not freeze. If longer delays are anticipated for tissue or fluid specimens (greater than 24 hours), specimens should be frozen at -70°C.
*Lynne S. Garcia, Editor-in-Chief. Specimen Collection, Transport, and Acceptability, Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press. Washington, D.C., 2010.
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.
This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills,
Grosse Pointe, Royal Oak, and Troy campuses.