ABO/Rh Type
ABO/D, ABO/Rh, Type & Group (Blood), Pediatric ABO/Rh Type, Newborn ABO/Rh Type, ABO/Rh Type, Rh (D) typing, ABO Type
Test Codes
ABO Group: EPIC: LAB5269, SOFT: ABO, ABO/Rh Type: EPIC: LAB5270, SOFT: TYPE, Rh Type: EPIC: LAB5273, SOFT: RH
Department
Blood Bank
Instructions
- Specimens used for transfusion purposes must be drawn within 3 days of intended transfusion. Day of collection is Day 0. Tubes drawn for cryoprecipitate, fresh frozen plasma, or platelet transfusions may be more than 3 days old, but must be obtained during the current admission/visit.
- For Pre-admission testing, one must order BOTH ABO/Rh Typing and Red Blood Cell Antibody Screen.
Specimen Collection Criteria
Collect: One Pink-top EDTA tube, properly labeled. (Minimum Volume: 2.0 mL for ABO/Rh Typing. If Antibody Screen is also needed, minimum volume is 5.0 mL.)
Also acceptable: (For Nursery Patients) One Pink-top EDTA Microtainer®, properly labeled. (Minimum Volume: 0.5 mL)
It is extremely important that blood samples are accurately labeled. Inpatient labels must include the following information:
- Patient's Name: Complete name, last name first. If the patient uses a first initial followed by a middle name, both must appear in proper order on the label.
- Wrist Band Number: Include the prefix letters, 4 numbers, and a suffix letter (example: BR1234T). Specimens lacking this complete information cannot be used for blood transfusion purposes.
- Hospital Identification Number: The medical record number must be included on the label.
- Date and Time Collected.
- Identification of Collector: Employee ID number must be on the sample tube or documented in the computer system.
Physician Office/Draw Specimen Preparation
Maintain specimen at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F) prior to transport.
Preparation for Courier Transport
Transport: Whole blood at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F).
Rejection Criteria
- Improperly labeled specimens.
- Specimens with inadequate volume.
- Specimens collected in inappropriate collectiontubes.
- Specimens contaminated with IV fluids.
- Grossly hemolyzed samples will be tested only in emergency situations.
In-Lab Processing
Centrifuge the specimen upon receipt in the Laboratory.
Storage
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 72 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): Specimens are held for a minimum of 7 days.
*Once a sample arrives in Blood Bank, it cannot be shared with other laboratories.
Laboratory
Royal Oak Transfusion Medicine Laboratory (Blood Bank)
Dearborn Transfusion Medicine Laboratory (Blood Bank)
Performed
Sunday – Saturday, 24 hours a day.
Results available within 2 hours of receipt in the Blood Bank for inpatient requests (without unexpected ABO discrepancies) and within 24 hours s after receipt in the Blood Bank for outpatient specimens.
Test Methodology
Hemagglutination.
Interpretation
By report.
Clinical Utility
The ABO system is the most important blood group antigen system in transfusion therapy. ABO and Rh typing is crucial to reduce the risk of transfusion reactions in patients receiving blood and blood components.
CPT Codes
86900 (ABO), 86901 (Rh).
Contacts
Blood Bank Laboratory – RO
248-898-9010
Name: Blood Bank Laboratory – RO
Location:
Phone: 248-898-9010
Blood Bank Laboratory – DBN
313-593-7915
Name: Blood Bank Laboratory – DBN
Location:
Phone: 313-593-7915
Last Updated
10/7/2023
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.