Bone Marrow Karyotype
Karyotyping, Chromosome Analysis, Karyogram, Bone Marrow, Bone Core Biopsy, genetic, GBMGT, cytogenetics
Test Codes
EPIC: LAB6460
Department
Cytogenetics
Specimen Collection Criteria
Collect: 2.0 mL bone marrow aspirate in a Dark Green-top Sodium Heparin tube. (Minimum: 1.0 mL)
- Bone marrow aspirates should be collected by a physician or trained technologist.
- Gently invert the tube to mix the specimen.
- A copy of the requisition must be sent with the specimen. The requisition form should contain the patient name, birth date, medical record number, requesting physician, specimen type and test request, date and time of collection, and tentative diagnosis.
- All specimens requiring FISH testing should identify the type of testing and tentative patient diagnosis on the requisition form. Both the FISH testing and conventional cytogenetics testing can be performed on the same specimen.
Physician Office/Draw Specimen Preparation
Do not freeze specimen. Maintain specimens at room temperature (20-25°C or 68-77°F) prior to courier pickup. For delays in transport (greater than 24 hours from the time of collection), refrigerate (2-8°C or 36-46°F) the specimen.
Preparation for Courier Transport
Transport: 2.0 mL bone marrow aspirate, at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)
Rejection Criteria
- Specimens arriving in the Laboratory 4 days or more following the original collection date.
- Clotted and sub-optimal specimens (volume less than 0.5 mL) will be cultured but the Laboratory cannot guarantee a final result.
Inpatient Specimen Preparation
Hematology will notify the Cytogenetics Laboratory of specimen pickups at extension 89030.
In-Lab Processing
Specimens are processed immediately upon delivery to the Cytogenetics Laboratory. Pediatric and STAT specimens may be given a priority in processing and analysis to provide a rush result.
Storage
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days. A backup cell pellet is maintained for three weeks past sign-out of the final report.
Laboratory
Royal Oak Cytogenetics Laboratory
Performed
Monday – Friday, 8:00 am – 5:00 pm.
Results available within 7-10 days of receipt in the Laboratory.
STAT analysis must be arranged in advance with the Laboratory.
Reference Range
Normal results would be 46,XX or 46,XY with no apparent abnormalities present.
Test Methodology
High resolution microscopic analysis of chromosomes to provide a digital image karyotype. Special stains or techniques such as C-Banding, NOR Banding and FISH may be required to further identify specific chromosomal abnormalities or variants.
Interpretation
Based on completion of chromosomal analysis. Interpretation will be issued in the form of a final report.
Clinical Utility
Karyotyping (chromosome analysis) is performed on oncologic specimens to determine the presence of chromosomal abnormalities. Bone marrow aspirates are obtained to identify and characterize chromosomal involvement in the disease process, to stage or confirm a diagnosis, and to provide post-treatment follow up information to physicians.
Clinical Disease
To aid in a complete treatment plan for patients, abnormal karyotype results may need to be followed up at a later date to aid in the staging and progression of the disease process.
CPT Codes
88237, 88262, 88280.
Contacts
Cytogenetics Laboratory – RO
248-898-9030
Name: Cytogenetics Laboratory – RO
Location:
Phone: 248-898-9030
Last Updated
12/30/2022
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