Human Herpesvirus 6 by PCR
Human herpesvirus 6 PCR, HHV-6, HHV6, Roseola, Herpesvirus-6 Human DNA DetectR, ARUP #60071, EPIC: LAB5913, SOFT: XHHVP
Specimen Collection Criteria
Collect (preferred specimen): One Lavender-top EDTA tube.
Also acceptable: One Gold-top SST tube, one plain Red-top tube, or Cerebrospinal Fluid (CSF) in a sterile collection container.
Physician Office/Drawsite Specimen Preparation
Centrifuge to separate serum or plasma from cells. Transfer serum or plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). Maintain CSF refrigerated prior to transport.
Preparation for Courier Transport
Transport: 1.0 mL serum, plasma, or CSF, refrigerated (2-8°C or 36-46°F). (Min: 0.5 mL)
- Specimens not collected and processed as indicated.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): 3 months
Specimen Storage in Department Prior to Disposal:
Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.
Sent to ARUP Laboratories, Salt Lake City, UT.
Tuesday - Saturday.
Results available in 2-5 days.
Reportable range for this assay is 1,000-999,000 HHV6 DNA copies/mL (3.0-6.0 log copies/mL).
Quantitative Polymerase Chain Reaction (PCR).
This assay aids in the diagnosis of roseola infantum (infection subitum) and other HHV-6 infections.
HHV-6 is the causative agent of roseola. Roseola is characterized by the abrupt onset of a high fever which is followed in 2-4 days by erthematous maculopapular rash that lasts for 1-2 days. Roseola lesions are macular or papular and never become vesicular like the lesions associated with VZV or HSV. Lesions typically appear on the neck and back and then spread to the abdomen and thighs. The syndrome is self-limiting but convulsions associated with high fever can cause permanent neurologic injury. Atypical roseola can occur in the absence of rash. (1)
HHV-6 infections are common and the virus has been found throughout the world. HHV-6 infections can occur in all age groups but they are usually acquired before three years of age. HHV-6 is the causative agent of roseola in children. Roseola is a common infection of infancy and the peak incidence of roseola occurs between the ages of 6 and 18 months. Infants less than 6 months are thought to be protected by maternal antibodies. Adults who become infected are often only mildly symptomatic. (1)
5-15 days. (1)
Transmission of HHV-6 is poorly understood. It is thought that the virus is transmitted after contact with oral secretions. (1)
- Locatelli G., Santoro F., Veglia F., Gobbi A., Lusso P and Malnati MS. 2000. Real-time quantitative PCR for human herpesvirus-6 DNA. J. Clin.Microbiology. 38: 4042-4048.
ARUP #60071, EPIC: LAB5913, SOFT: XHHVP