Beaumont Laboratory - Lab Test Details
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Beaumont Laboratory

Transplant Monitoring Panel

Transplant Monitoring Panel, OKT-3, Flow Cytometry, Transplant Monitoring Panel, EPIC: LAB6412, SOFT: FOKTG

Instructions

A Complete Blood Count and Differential (CBCD) must be ordered simultaneously to obtain the absolute lymphocyte count.

Specimen Collection Criteria

Collect: One Lavender-top EDTA tube for Transplant Monitoring Panel AND one Lavender-top EDTA tube for Complete Blood Count with Differential. (Minimum: 2.0 mL each tube.)

Tube should be labeled with the date of time of collection.

Physician Office/Drawsite Specimen Preparation

Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Specimens must be received in the Laboratory within 48 hours of collection.

Preparation for Courier Transport

Transport: Whole blood, at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

  • Clotted specimens.
  • Refrigerated specimens.
  • Specimens received greater than 48 hours post collection.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Flow Cytometry Laboratory.

Performed

Monday - Saturday.
Results available in 1-2 days.

Reference Range

CD2: 660-2769 mill/L.
CD3: 581-2581 mill/L.

Test Methodology

Immunophenotyping by Multiparameter Flow Cytometry.

Interpretation

This assay includes: absolute lymphocyte count, CD3 (absolute and %), and CD2 (absolute and %).

Clinical Utility

OKT3 is a murine monoclonal antibody directed against the CD3 antigen on lymphocytes that closely associates with the T-cell receptor for antigen. It is remarkably effective in reversing or preventing rejection episodes because it clears the circulation of CD3 positive cells and prevents allograft antigen recognition. Effective therapy with OKT3 can be verified by documenting CD3 antigen removal.

CPT Code

88184x1, 88185x2, 88187 - Interpretation 2-8 markers.

Test Codes

EPIC: LAB6412, SOFT: FOKTG

Last Updated

08/29/2017

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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.