Lab results are too important to go anywhere else

Beaumont Laboratory

Vitamin D, 25 Hydroxy

Antrim #30775, EPIC: LAB5845, SOFT: D25OH

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)

Physician Office/Drawsite Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Min: 1.0 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Min: 1.0 mL)

Rejection Criteria

  • Plasma specimens.
  • Hemolyzed specimens.
  • Severely lipemic specimens.


Specimen Stability for Testing:

Centrifuged SST Tubes, Red-top Tubes, and Microtainers®
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days


Farmington Hills Chemistry Laboratory.
Royal Oak Special Testing Laboratory.


Farmington Hills

Results available within 24 hours.

Royal Oak

Monday - Friday.
Results available within 2 business days.

Reference Range

Less than 10 ng/mL (severe deficiency).
10-24 ng/mL (mild to moderate deficiency).
25-80 ng/mL (optimum levels).
Greater than 80 ng/mL (toxicity possible).

Test Methodology

Direct Competitive Chemiluminescence Immunoassay (CLIA).


Recent literature suggests that optimal bone health is achieved when the circulating 25-OH vitamin D level is maintained at levels greater than 32 ng/mL.

Patients with 25-OH vitamin D less than 25 ng/mL have an increased risk of secondary hyperparathyroidism, reduced bone mineral density and fractures. Levels less than 10 ng/mL are associated with more severe abnormalities including inadequate mineralization (rickets in children and osteomalacia in adults). Toxic effects may be seen with serum 25-OH vitamin D levels greater than 80 ng/mL although they are more likely to be encountered when the level is greater than 150 ng/mL.

Although 25-OH vitamin D is not the active hormone it is the most useful form of vitamin D for assessment of vitamin D stores. Levels below 25 ng/mL are now considered to be low.

Clinical Utility

This assay is used to diagnose vitamin D deficiency and aids in the differential diagnosis of hypo and hypercalcemia. Increased vitamin D levels may lead to hypertension, nephrolithiasis, and metastatic calcifications.

CPT Code


Test Codes

Antrim #30775, EPIC: LAB5845, SOFT: D25OH

Last Updated


Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.

This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.