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Beaumont Laboratory

HIV-1 RNA Qualitative by PCR

Human Immunodeficiency Virus 1 quantitation, HIV 1 RNA Qualitative By PCR, EPIC: LAB7097, SOFT: IHIVR

Specimen Collection Criteria

Collect: Two Lavender-top EDTA tubes.

  • Minimum volume and pediatric minimum (single test with no repeat): 2.5 mL whole blood (1.1 mL plasma).
  • Separate samples must be submitted when multiple tests are ordered.

Physician Office/Drawsite Specimen Preparation

Centrifuge to separate plasma from cells within 24 hours of collection. Transfer all available plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). (Min: 1.1 mL)

Preparation for Courier Transport

Transport: Plasma, refrigerated (2-8°C or 36-46°F). (Min: 1.1 mL)

Rejection Criteria

  • Specimens not centrifuged within 24 hours of collection.
  • Unlabeled tubes.
  • Specimens not collected in Lavender-top EDTA tubes.
  • Samples exposed to repeated freeze/thaw cycles.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): 4 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days


Royal Oak Clinical Molecular Pathology Laboratory.


Once per week
Results available within 7-10 days.

Reference Range

HIV-1 RNA not detected. The limit of detection is 20 copies/mL.

Test Methodology

Real-time polymerase chain reaction: Roche COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, v2.0 (FDA Approved).

Clinical Utility

This test is useful to determine the presence or absence of HIV-1 RNA in peripheral blood.  Test results should be interpreted in conjunction with clinical and other laboratory data of the patient.

Disease Reporting

This is a reportable infection and positive results will be reported to the Oakland County Health Department. For more information on reportable diseases, contact the Epidemiology Department at (248) 551-4040.

CPT Code


Test Codes


Last Updated


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This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.