Haemophilus Influenzae B IgG
Haemophilus Influenzae Type b Antibody, IgG , ARUP #50542, Antrim #81050, EPIC: LAB6045, SOFT: XHAIG
"Pre" and "post" Haemophilus influenzae B vaccination samples should be submitted together for testing. "Post" samples should be drawn 30 days after immunization and must be received within 60 days of "pre" sample. Please clearly mark samples "pre-vaccine" or "post-vaccine" so that samples will be saved and tested simultaneously.
Specimen Collection Criteria
Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube.
Physician Office/Drawsite Specimen Preparation
Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).
Preparation for Courier Transport
Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Min: 0.15 mL)
- Hemolyzed specimens.
- Severely lipemic specimens.
- Specimens not collected and processed as indicated.
After separation from clot:
Ambient: 2 days
Refrigerated: 2 weeks
Frozen: 1 year (avoid repeat freeze/thaw cycles)
Sunday - Saturday.
Results available in 2-4 days.
Less than 1.0 mcg/mL: Antibody concentration not protective.
Greater than or equal to 1.0 mcg/mL: Antibody to Haemophilus influenzae B detected. Suggestive of protection.
Quantitative Multiplex Bead Assay.
Responder status is determined according to the ratio of post-vaccination concentration to pre-vaccination concentration of Haemophilus influenza B antibody, IgG as follows:
- If the post-vaccination concentration is less than 3.0 mcg/mL, the patient is considered to be a non-responder.
- If the post-vaccination concentration is greater than or equal to 3.0 mcg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a non-responder.
This assay is used to assess the production of antibody against a protein-polysaccharide conjugate vaccine.
ARUP #50542, Antrim #81050, EPIC: LAB6045, SOFT: XHAIG