Details

HDL Cholesterol

EPIC: LAB5070, SOFT: HDLC

Instructions

It is preferable that the patient fast for 9-12 hours prior to blood draw, but it is not mandatory.

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)
Contact Laboratory for acceptability of other tube types.

Physician Office/Drawsite Specimen Preparation

Let SST specimens clot for 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection. (Minimum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged SST tube, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL)

Rejection Criteria

  • Moderate to grossly hemolyzed specimens.
  • Red-top tubes with serum not separated from cells within two hours of collection.

Storage

Centrifuged SST tubes and Microtainers® with separator gel may be transported and stored at room temperature (up to 25oC or 77oF) for 2-4 hours. DO NOT FREEZE TUBES CONTAINING WHOLE BLOOD. For longer storage, the specimen should be refrigerated (2o-8oC).

Red-top tubes and Microtainers® without separator gel may be stored at room temperature for up to 2 hours. DO NOT FREEZE TUBES CONTAINING WHOLE BLOOD. For longer storage, the serum should be removed from the clot and refrigerated (2o-8oC).

Serum specimens or (pour-overs) may be stored at room temperature (up to 25oC or 77oF) for 2-4 hours. For longer storage the sample should be refrigerated (2o to 8oC; 36o to 47o F) for one week.

Performed

Sunday – Saturday, 24 hours a day.
Routine results available within 4 hours.

Reference Range

Grosse Pointe, Royal Oak & Troy:

0-19 Years Old:
 35-90 mg/dL.
Greater than 19 Years Old: 40-90 mg/dL.

Farmington Hills:

0-19 Years Old:
 Greater than equal to 35 mg/dL.
Greater than 19 Years Old: Greater than equal to 40 mg/dL

Test Methodology

Enzymatic, Spectrophotometry.

Interpretation

Elevated HDL-cholesterol concentrations are generally protective against coronary heart disease. Decreased HDL-cholesterol concentrations, particularly in conjunction with elevated triglycerides, are associated with an increased cardiovascular disease risk. Factors contributing to a decreased HDL cholesterol include obesity, lack of exercise, cigarette smoking, steroids, hypertriglyceridemia and genetic factors. Tangier disease is a rare inherited disorder characterized by very low HDL, total cholesterol and LDL cholesterol levels.

Clinical Utility

The quantitative determination of HDL (high-density lipoprotein) cholesterol aids in the diagnosis and treatment of lipid disorders. It should be used in the risk assessment of coronary artery disease (CAD). Low levels of HDL cholesterol are associated with an increased risk of CAD.

CPT Code

83718

Test Codes

EPIC: LAB5070, SOFT: HDLC

Last Updated

7/11/2019