Histone Antibody (IgG)
Anti-Histone Antibody , Antrim #30375, EPIC: LAB5761, SOFT: HISG
Specimen Collection Criteria
Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)
Physician Office/Drawsite Specimen Preparation
Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)
Preparation for Courier Transport
Transport: Centrifuged collection tube, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 0.5 mL)
- Plasma specimens.
- Severely hemolyzed, lipemic or icteric specimens.
0,Centrifuged SST tubes may be transported
and stored at ambient temperatures (2o to 30oC; 36 to 86oF)
for up to 48 hours. DO NOT FREEZE TUBES CONTAINING BLOOD. For
longer storage, the serum should be removed from the gel and refrigerated
(2o-8oC) or frozen at -20oC or below.
Red-top tubes and Microtainers® may be stored at ambient temperatures
for up to 16 hours and for up to 3 days at refrigerator temperatures (2o-8oC).
DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should
be removed from the clot and refrigerated (2o-8oC) or frozen at
-20oC or below.
0,Serum specimens (pour-overs) may be stored at room temperature
(20o to 26oC; 68 to 79oF) for up to 1 week, refrigerator temperature
(2 to 8oC; 36 to 47oF) for two weeks, and at -20oC (-4oF)
for up to 3 months. Specimens stored at -70oC (- 94oF) can
be stored indefinitely.
Monday – Friday.
Results available within 2 business days.
Negative: Less than 100 AU/mL.
Equivocal: 100-120 AU/mL.
Positive: Greater than 120 AU/mL.
Multiplex Bead Immunoassay.
Histone antibodies are present in 20-55% of idiopathic SLE and 80-95% in drug induced SLE. They occur in less than 20% of other types of connective tissue diseases.
The histone antibody assay aids in the diagnosis of SLE.
Antrim #30375, EPIC: LAB5761, SOFT: HISG