HIV-1 RNA Qualitative by PCR

Human Immunodeficiency Virus 1 quantitation, HIV 1 RNA Qualitative By PCR , EPIC: LAB7097, SOFT: IHIVR

Specimen Collection Criteria

Collect: Two Lavender-top EDTA tubes.

  • Minimum volume and pediatric minimum (single test with no repeat): 2.5 mL whole blood (1.1 mL plasma).
  • Separate samples must be submitted when multiple tests are ordered.

Physician Office/Drawsite Specimen Preparation

Centrifuge to separate plasma from cells within 24 hours of collection. Transfer all available plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F). (Min: 1.1 mL)

Preparation for Courier Transport

Transport: Plasma, refrigerated (2-8°C or 36-46°F). (Min: 1.1 mL)

Rejection Criteria

  • Specimens not centrifuged within 24 hours of collection.
  • Unlabeled tubes.
  • Specimens not collected in Lavender-top EDTA tubes.
  • Samples exposed to repeated freeze/thaw cycles.


Once per week
Results available within 7-10 days.

Reference Range

HIV-1 RNA not detected. The limit of detection is 20 copies/mL.

Test Methodology

Real-time polymerase chain reaction: Roche COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, v2.0 (FDA Approved).

Clinical Utility

This test is useful to determine the presence or absence of HIV-1 RNA in peripheral blood.  Test results should be interpreted in conjunction with clinical and other laboratory data of the patient.

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Test Codes


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