Details

Hepatitis B Core Antibody IgM (HBc IgM)

HBcIgM, Hepatitis B Core Antibody, IgM (HBcIgM) , Antrim #30960, EPIC: LAB5219, SOFT: HBCM

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)
Also acceptable: One Lavender-top EDTA tube. (Minimum Whole Blood: 3.0 mL) 

Note: Patient Preparation (Royal Oak ONLY): Twenty-four (24) hours before this test, the patient should not take multivitamins or dietary supplements containing biotin (vitamin B7) which is commonly found in hair, skin and nail supplements and multivitamins.

Physician Office/Drawsite Specimen Preparation

  • Let SST specimens clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection. (Min: 2.0 mL)
  • Centrifuge Lavender-top tube immediately to separate plasma from cells. Transfer plasma to a plastic transport tube and refrigerate (2-8°C or 36-46°F) within twelve hours of collection. (Min: 2.0 mL) 

Preparation for Courier Transport

Transport: Centrifuged SST tube or plasma aliquot, refrigerated (2-8°C or 36-46°F). (Min: 2.0 mL serum or plasma)

Rejection Criteria

  • Hemolyzed specimens.
  • Severely lipemic specimens.
  • Red-top tubes with serum not separated from cells within two hours of collection.
  • Lavender-top tubes with plasma not separated from cells within twelve hours of collection. 

Storage

Centrifuged SST tubes may be transported and stored at ambient temperatures (2o to 30oC; 36o to 86oF) for up to 48 hours. DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the gel and refrigerated (2o-8oC) or frozen at -20o C or below.

Red-top tubes and Microtainers® may be stored at ambient temperatures for up to 16 hours and for up to 3 days at refrigerator temperatures (2o -8oC).DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the clot and refrigerated (2o-8oC) or frozen at -20o C or below.

Lavender top tubes can be stored at ambient temperatures (2o to 30oC; 36o to 86oF) for up to 12 hours.

Serum and plasma (pour-overs) may be stored at room temperature (20o to 26o C; 68o to 79o F) for up to 5 days, refrigerator temperature (2o to 8oC; 36o to 47o F) for two weeks, and at -20oC (-4o F) for up to 3 months. Specimens stored at -70o C (-94o F) can be stored indefinetly.

Performed

Sunday - Saturday, 24 hours a day.
Results available within 24 hours of receipt in the Laboratory.

Reference Range

Negative/Non-reactive.

Test Methodology

Chemiluminescence Immunoassay.

Interpretation

  • This assay cannot be used to determine a patient's immune status to hepatitis B.
  • Patients with Equivocal/Borderline result should be redrawn at approximately one week intervals to distinguish rising IgM anti-HBc levels (associated with acute hepatitis B infection) from falling or unchanging IgM anti-HBc levels (associated with recovery).

Clinical Utility

  • This assay aids in the diagnosis of acute or recent (usually six months or less) hepatitis B viral infection.
  • IgM anti-HBc arises early in the illness of patients with acute hepatitis B, but it rapidly decreases in titer. HBV core IgM levels are generally not detectable 6 - 24 months after the onset of illness. (1)
  • The incubation period for hepatitis B is approximately 70 days (range, 30 - 180 days) (1).

Clinical Disease

Patients with hepatitis B may present with fatigue, poor appetite, fever, vomiting and occasionally joint pain, hives or rash. Urine may become darker in color, and then jaundice (a yellowing of the skin and whites of the eyes) may appear. Patients may also be asymptomatic or experience only a few symptoms.

Reference

  1. Hsu, H., Feinstone, S., Hoofnagle, J. Acute Viral Hepatitis in Mandell, G., Bennett, J., Dolin. R.(eds.), Principles of Infectious Diseases 7th edition New York,N.Y. 2005 Chapter 115,116.
  2. Farrar, W., Wood, M., Innes, J., Tubbs, H. Infectious Diseases, Time Mirror International Publishers Ltd New York, N.Y. 1995.

CPT Code

86705.

Test Codes

Antrim #30960, EPIC: LAB5219, SOFT: HBCM

Last Updated

7/11/2019