Details

Hepatitis B Core Antibody Total (HBc T)

HBcAb, Anti-HBc, Antibody to Hepatitis B Core Antigen, Hepatitis B Core Antibody Total (HBcAb) , Antrim #30100, EPIC: LAB5220, SOFT: HBCT

Instructions

Hepatitis B Core Antibody (Total) measures both IgG and IgM antibodies but does not differentiate between them.

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)
Also acceptable: One Lavender-top EDTA tube. (Minimum Whole Blood: 3.0 mL)

Contact the Laboratory for the acceptability of other tube types.

Physician Office/Drawsite Specimen Preparation

Let SST specimens clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection.

Centrifuge Lavender-top tube immediately to separate plasma from cells. Transfer 3.0 mL plasma to plastic transport tube and refrigerate (2-8°C or 36-46°F) within two hours of collection.

Preparation for Courier Transport

Transport: Centrifuged SST tube or 2.0 mL plasma, refrigerated (2-8°C or 36-46°F). (Min: 2.0 mL serum or plasma)

Rejection Criteria

  • Severely lipemic or hemolyzed specimens.
  • Red-top tubes with serum not separated from cells within two hours of collection. 

Storage

Centrifuged SST tubes may be transported and stored at ambient temperatures (2o to 30oC; 36o to 86oF) for up to 48 hours. DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the gel and refrigerated (2o-8oC) or frozen at -20o C or below.

Red-top tubes and Microtainers® may be stored at ambient temperatures for up to 16 hours and for up to 3 days at refrigerator temperatures (2o -8oC).DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage, the serum should be removed from the clot and refrigerated (2o-8oC) or frozen at -20o C or below.

Lavender top tubes can be stored at ambient temperatures (2o to 30oC; 36o to 86oF) for up to 12 hours.

Serum and plasma (pour-overs) may be stored at room temperature (20o to 26o C; 68o to 79o F) for up to 5 days, refrigerator temperature (2o to 8oC; 36o to 47o F) for two weeks, and at -20oC (-4o F) for up to 3 months. Specimens stored at -70o C (-94o F) can be stored indefinetly.

Performed

Sunday - Saturday, 24 hours a day.
Results available within 24 hours of receipt in the Laboratory.

Reference Range

Negative.

Test Methodology

Chemiluminescence Immunoassay.

Interpretation

This assay tests for IgG and IgM antibodies but does not differentiate between them.

Clinical Utility

A positive result indicates acute, recent, resolving or past infection with hepatitis B. Both total and IgM hepatitis B core antibody will be positive in the “core window” when hepatitis B surface Ag is negative.

Clinical Disease

Patients with hepatitis B may present with fatigue, poor appetite, fever, vomiting and occasionally joint pain, hives or rash. Urine may become darker in color, and then jaundice (a yellowing of the skin and whites of the eyes) may appear. Patients may also be asymptomatic or experience only a few symptoms. The incubation period for hepatitis B is approximately 70 days (range, 30-180 days). (1)

Reference

  1. Hsu, H., Feinstone, S., Hoofnagle, J. Acute Viral Hepatitis in Mandell, G., Bennett, J., Dolin, R. (eds.), Principles of Infectious Diseases 7th edition New York, N.Y. 1995; 2005 Chapter 115,116.
  2. Farrar, W., Wood, M., Innes, J., Tubbs, H. Infectious Diseases, Time Mirror International Publishers Ltd New York, N.Y. 1995.

CPT Code

86704.

Test Codes

Antrim #30100, EPIC: LAB5220, SOFT: HBCT

Last Updated

7/11/2019