Hepatitis B Core Antibody Total (HBc T)
HBcAb, Anti-HBc, Antibody to Hepatitis B Core Antigen, Hepatitis B Core Antibody Total (HBcAb) , Antrim #30100, EPIC: LAB5220, SOFT: HBCT
Hepatitis B Core Antibody (Total) measures both IgG and IgM antibodies but does not differentiate between them.
Specimen Collection Criteria
Collect (preferred specimen): One Gold-top SST tube. (Minimum Whole Blood: 4.0 mL)
Also acceptable: One Lavender-top EDTA tube. (Minimum Whole Blood: 3.0 mL)
Contact the Laboratory for the acceptability of other tube types.
Physician Office/Drawsite Specimen Preparation
Let SST specimens clot 30-60 minutes then immediately centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged SST tube within two hours of collection.
Centrifuge Lavender-top tube immediately to separate plasma from cells. Transfer 3.0 mL plasma to plastic transport tube and refrigerate (2-8°C or 36-46°F) within two hours of collection.
Preparation for Courier Transport
Transport: Centrifuged SST tube or 2.0 mL plasma, refrigerated (2-8°C or 36-46°F). (Min: 2.0 mL serum or plasma)
- Severely lipemic or hemolyzed specimens.
- Red-top tubes with serum not separated from cells within two hours of collection.
Centrifuged SST tubes may be transported
and stored at ambient temperatures (2o to 30oC;
36o to 86oF) for up to 48 hours. DO NOT FREEZE TUBES
CONTAINING BLOOD. For longer storage, the serum should be removed from
the gel and refrigerated (2o-8oC) or frozen at -20o
C or below.
Red-top tubes and Microtainers® may be stored at ambient temperatures
for up to 16 hours and for up to 3 days at refrigerator temperatures (2o
-8oC).DO NOT FREEZE TUBES CONTAINING BLOOD. For longer storage,
the serum should be removed from the clot and refrigerated (2o-8oC)
or frozen at -20o C or below.
Lavender top tubes can be stored at ambient temperatures (2o
to 30oC; 36o to 86oF) for up to 12 hours.
Serum and plasma (pour-overs) may be stored
at room temperature (20o to 26o C; 68o
to 79o F) for up to 5 days, refrigerator temperature (2o
to 8oC; 36o to 47o F) for two weeks, and
at -20oC (-4o F) for up to 3 months.
Specimens stored at -70o C (-94o F) can be stored
Sunday - Saturday, 24 hours a day.
Results available within 24 hours of receipt in the Laboratory.
This assay tests for IgG and IgM antibodies but does not differentiate between them.
A positive result indicates acute, recent, resolving or past infection with hepatitis B. Both total and IgM hepatitis B core antibody will be positive in the “core window” when hepatitis B surface Ag is negative.
Patients with hepatitis B may present with fatigue, poor appetite, fever, vomiting and occasionally joint pain, hives or rash. Urine may become darker in color, and then jaundice (a yellowing of the skin and whites of the eyes) may appear. Patients may also be asymptomatic or experience only a few symptoms. The incubation period for hepatitis B is approximately 70 days (range, 30-180 days). (1)
- Hsu, H., Feinstone, S., Hoofnagle, J. Acute Viral Hepatitis in Mandell, G., Bennett, J., Dolin, R. (eds.), Principles of Infectious Diseases 7th edition New York, N.Y. 1995; 2005 Chapter 115,116.
- Farrar, W., Wood, M., Innes, J., Tubbs, H. Infectious Diseases, Time Mirror International Publishers Ltd New York, N.Y. 1995.
Antrim #30100, EPIC: LAB5220, SOFT: HBCT